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CD4 Intracellular Cytokine Detection Kit Anti-Hu-TNF-α/CD69/CD4

BD FastImmune™ CD4 Intracellular Cytokine Detection Kit Anti-Hu-TNF-α/CD69/CD4

Product Details
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BD FastImmune™
Human
Intracellular staining (flow cytometry)
RUO (GMP)


Description

The BD FastImmune™ CD4 Intracellular Cytokine Detection Kit is designed for the detection of intracellular cytokines and the activation marker CD69 in antigen-activated CD4+ T lymphocytes in whole blood. Applications include studies of T-cell responses to antigens, such as herpes viruses, HIV, and tumor antigens.

Contents: Antibody Cocktail (TNF-α FITC, CD69 PE, CD4 PerCP-Cy™5.5), Isotype Ctrl Cocktail, Co-Stimulatory Antibodies (CD28/CD49d), Brefeldin A (BFA) Solution, EDTA Solution, BD FACS™ Lysing Solution (10x), BD FACS Permeabilizing Solution 2 (10x).

Preparation And Storage

Upon receipt, thaw BFA, dispense into 10 µL aliquots, and store at –20°C.

Store each kit at 2° to 8°C.

BD FACS Lysing Solution (10X) and BD FACS Permeabilizing Solution 2 (10X) are each stable for the period shown on the appropriate bottle label when stored as directed. Do not use either reagent if discoloration occurs or a precipitate forms. Dilute 1:10 in deionized (DI) water. Use at room temperature.

When stored at 2° to 8°C, each antibody reagent is stable until the expiration date shown on the label. Do not use after the expiration date. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Alteration in the appearance of the reagent, such as precipitation or discoloration, indicates instability or deterioration. In such cases, the reagent should not be used.

340972 Rev. 1
Components
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Description Quantity/Size Part Number EntrezGene ID
N/A 25.0 N/A N/A
340972 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures. 

 

Although not required, these products are manufactured in accordance with Good Manufacturing Practices.