BD Multitest™ Anti-Human CD45RA FITC/CD45RO PE/CD3 PerCP/CD4 APC with BD Trucount™ Tubes
Description
CD45RA, clone L48, is derived from hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c mice immunized with low-buoyant–density human lymphocytes. CD45RO, clone UCHL-1, is derived from hybridization of mouse P3/NS-1/1-Ag4-1 myeloma cells with spleen cells from BALB/c mice immunized with an interleukin-2 (IL-2)-dependent human T-cell line. CD3, clone SK7, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human thymocytes. CD4, clone SK3, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood T lymphocytes. CD45RA recognizes an Mr 220-kDalton (kDa) isoform of the leucocyte common antigen (LCA). The CD45RA antigen is a member of the CD45 antigen family that also includes the CD45, CD45RB, and CD45RO antigens. CD45RO, a member of the same CD45 family as CD45RA, recognizes a 180-kDa isoform of the LCA. The CD45 antigen is a protein tyrosine phosphatase. CD3 recognizes the epsilon chain of the CD3 antigen/T-cell antigen receptor (TCR) complex. This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kDa. The antigen recognized by the CD3 antibody is noncovalently associated with either α/β or γ/δ TCR (70 to 90 kDa). CD4 recognizes an antigen that interacts with class II molecules of the major histocompatibility complex and is the primary receptor for the human immunodeficiency virus. The cytoplasmic portion of the antigen is associated with the protein tyrosine kinase p56lck. The CD4 antigen can regulate the function of the CD3 antigen/TCR complex. CD4 also reacts with monocytes/macrophages that have an antigen density lower than that on helper/inducer T lymphocytes.
Preparation And Storage
The MultiTEST reagent is supplied as a combination of CD45RA FITC, CD45RO PE, CD3 PerCP, and CD4 APC in 1.0 mL of PBS containing bovine serum albumin, and 0.1% sodium azide. Vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.
| Description | Clone | Isotype | EntrezGene ID |
|---|---|---|---|
| N/A | N/A | IgG1, κ | N/A |
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Although not required, these products are manufactured in accordance with Good Manufacturing Practices.