The s19.8 monoclonal antibody specifically recognizes the b and c alloantigens of β2 microglobulin, originally designated Ly-m11. β2 microglobulin is a 12 kDa protein, homologous to a single immunoglobulin constant-region domain, which is noncovalently associated with the MHC class I heavy chain. It may also associate noncovalently with CD1d in some cells types. Positive strains include C57BL/6, C57BL/10, C57BR, and C57L; whereas, A, AKR, BALB/c, C58, C3H, CBA, DBA/1, DBA/2, SJL, SWR, and 129 strains are negative.
The antibody was conjugated to an oligonucleotide that contains an antibody clone-specific barcode (ABC) flanked by a poly-A tail on the 3' end and a PCR handle (PCR primer binding site) on the 5' end. The ABC for this antibody was designed to be used with other BD AbSeq oligonucleotides conjugated to other antibodies. All AbSeq ABC sequences were selected in silico to be unique from human and mouse genomes, have low predicted secondary structure, and have high Hamming distance within the BD AbSeq portfolio, to allow for sequencing error correction and unique mapping. The poly-A tail of the oligonucleotide allows the ABC to be captured by the BD Rhapsody™ system. The 5' PCR handle allows for efficient sequencing library generation for Illumina sequencing platforms.
NOTE: The BD Rhapsody Single-Cell Analysis System must be used with the BD Rhapsody Express Instrument.