Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
BMX (bone marrow X kinase) is a cytoplasmic tyrosine kinase identified by reverse transcription of mRNA isolated from human bone marrow and mapped to the chromosomal band Xp22.2. The full length protein is 675 amino acids with a tyrosine kinase domain, an amino terminal pleckstrin domain, as well as an SH3 and SH2 domain. Direct comparison of BMX's primary sequence with other kinases showed that this is highly related to the family of BTK/ITK/TEC. BMX kinase is expressed in fetal and adult tissues, with the highest expression in heart, testis, small intestine and colon. It is undetectable in spleen, brain, kidney, and pancreas. Further analysis of mRNA expression showed that BMX is expressed in hematopoietic tissues and neutrophilic granulocytes, and in patients with acute and myeloid leukemia. The levels of BMX mRNA were substantially lower in patients with acute and chronic lymphoid leukemias, thus suggesting that BMX may be important during myelopoiesis.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (4)
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Kaukonen J, Lahtinen I, Laine S, Alitalo K, Palotie A. BMX tyrosine kinase gene is expressed in granulocytes and myeloid leukaemias. Br J Haematol. 1996; 94(3):455-460. (Biology). View Reference
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Rajantie I, Ekman N, Iljin K. Bmx tyrosine kinase has a redundant function downstream of angiopoietin and vascular endothelial growth factor receptors in arterial endothelium. Mol Cell Biol. 2001; 21(14):4647-4655. (Clone-specific: Western blot). View Reference
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Tamagnone L, Lahtinen I, Mustonen T. BMX, a novel nonreceptor tyrosine kinase gene of the BTK/ITK/TEC/TXK family located in chromosome Xp22.2. Oncogene. 1994; 9(12):3683-3688. (Biology). View Reference
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Vargas L, Nore BF, Berglof A. Functional interaction of caveolin-1 with Bruton's tyrosine kinase and Bmx. J Biol Chem. 2002; 277(11):9351-9357. (Clone-specific: Immunoprecipitation, Western blot). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.