Alexa Fluor® 488 Mouse anti-SSEA-1
Immunofluorescent staining of mouse ES cell line. ES-E14TG2a cells (ATCC CRL-1821) were cultured, fixed, and stained with Alexa Fluor® 488 Mouse anti-SSEA-1 (pseudo-colored green) according to the Recommended Assay Procedure. Counter-staining was with Hoechst 33342 (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer using a 20X objective and merged using BD Attovision™ software. This antibody also stained F9 cells (ATCC CRL-1720; mouse embryonal carcinoma). Methanol and Triton X100 permeabilization agents are not recommended for use with this antibody.
BD Pharmingen™ Alexa Fluor® 488 Mouse anti-SSEA-1
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