Alexa Fluor® 488 Mouse anti-Human TRA-1-60 Antigen
Immunofluorescent staining of human ES cell line. The H9 cell line (WiCell, Madison, WI) was cultured, fixed, and stained with Alexa Fluor® 488 Mouse anti-Human TRA-1-60 Antigen monoclonal antibody (pseudo-colored green) according to the Recommended Assay Procedure. The left image shows the plasma membrane staining by the TRA-1-60 mAb, and the right image shows TRA-1-60 with counter-staining of the nuclei by Hoechst 33342 (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer using a 10X objective and merged using BD Attovision™ software. If permeabilization is required for staining additional markers, we recommend the use of cold 90% methanol or BD™ Phosflow Perm Buffer III.
BD Pharmingen™ Alexa Fluor® 488 Mouse anti-Human TRA-1-60 Antigen
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