Description
Interleukin-6 (IL-6, aka IFN-β2, BSF-2, and HPGF) is a multifunctional cytokine which regulates immune responses, acute-phase reactions and hematopoiesis. Reported cellular targets for IL-6 action are hepatocytes, B and T lymphocytes, neurons, tumor cells and multi-potent hematopoietic cells. Recombinant rat IL-6 (Cat. No. 557008) is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution, glycerol and bovine serum albumin, with no preservatives. Recombinant rat IL-6 is ≥ 95% pure as determined by SDS-PAGE and an absorbance assay based on the Beers-Lambert law. The endotoxin level is ≤ 0.1 ng per μg of rat IL-6, as measured in a chromogenic LAL assay.
Preparation And Storage
Recommended Assay Procedures
Upon initial thawing, recombinant rat IL-6 (Cat. No. 557008) should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 – 1.0 mg/mL carrier protein, such as human or bovine serum albumin, aliquoted and stored at -80°C. Failure to add carrier protein or store at the indicated temperatures may result in a loss of activity. Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may have an undesired influence on experimental results due to toxicity, high endotoxin levels or possible blocking activity.
ELISA Standard: Rat IL-6 is useful as a quantitative standard for measuring rat IL-6 protein levels in a IL-6 specific sandwich ELISA, with the purified C3-4 antibody (Cat. No. 550644) as a capture antibody and the biotinylated G307-2 antibody (Cat. No. 550642) as the detection antibody. This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems, not for assay of serum or plasma samples. For measuring rat IL-6 in serum or plasma, the BD OptEIA™ Rat IL-6 Set (Cat. No. 550319) is specially formulated and recommended.
Bioassay: Investigators are advised that the Bioassay application is not routinely tested for this material and are highly encouraged to both titrate this material and include appropriate controls in relevant experiments. An activity range of 1.0 - 6.6 x 10^7 units/mg, encompassing an ED50= 0.15 - 1.0 ng/mL, has previously been reported using NFS-60 indicator cells for proliferation, with a unit defined as the amount of material needed to stimulate a half-maximal response at cytokine saturation.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Development References (5)
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Chiu CP, Moulds C, Coffman RL, Rennick D, Lee F. Multiple biological activities are expressed by a mouse interleukin 6 cDNA clone isolated from bone marrow stromal cells. Proc Natl Acad Sci U S A. 1988; 85(19):7099-7103. (Biology). View Reference
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Van Snick J, Cayphas S, Szikora JP, et al. cDNA cloning of murine interleukin-HP1: homology with human interleukin 6. Eur J Immunol. 1988; 18(2):193-197. (Biology). View Reference
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Van Snick J, Cayphas S, Vink A, et al. Purification and NH2-terminal amino acid sequence of a T-cell-derived lymphokine with growth factor activity for B-cell hybridomas. Proc Natl Acad Sci U S A. 1986; 83(24):9679-9683. (Biology). View Reference
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Van Snick J. Interleukin-6: an overview. Annu Rev Immunol. 1990; 8:253-278. (Biology). View Reference
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Weinstein Y, Ihle JN, Lavu S, Reddy EP. Truncation of the c-myb gene by a retroviral integration in an interleukin 3-dependent myeloid leukemia cell line. Proc Natl Acad Sci U S A. 1986; 83(14):5010-5014. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.