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Purified Rat Anti-Mouse IgG1
Product Details
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BD Pharmingen™
Mouse (QC Testing)
Rat IgG1, κ
Pooled mouse IgG1
ELISA (Routinely Tested)
0.5 mg/ml
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

ELISA:  The Purified Rat Anti-Mouse IgG1 antibody (clone A85-3) may be used as a capture reagent with Biotin Rat Anti-Mouse IgG1 (clone A85-1) (Cat. No. 553441) and Avidin-HRP (Cat. No. 554058) or Streptavidin-HRP (Cat. No. 554066) as detection reagents.  Alternatively,  investigators may also wish to consider using AKP Rat Anti-Mouse IgG1 (Cat. No. 557272) or HRP Rat Anti-Mouse IgG1 (Cat. No. 559626) for detection.  Purified Mouse IgG1 (Cat. No. 557273) should be used as the protein standard.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  4. Please refer to for technical protocols.
553445 Rev. 9
Antibody Details
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The A85-3 antibody reacts specifically with mouse IgG1 of Igh-C[a] and Igh-C[b] haplotypes. It does not react with other Ig isotypes.

553445 Rev. 9
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
553445 Rev.9
Citations & References
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553445 Rev. 9

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.