Preparation And Storage
Recommended Assay Procedures
This antibody is useful as a standard in ELISA or as an isotype-matched negative control for immunostaining. An isotype control should be used at the same concentration as the antibody of interest (e.g., ≤ 1 µg/million cells for flow cytometry).
For immunohistochemical staining, we recommend the use of purified MOPC-31C mAb in our special formulation for immunohistochemistry (Cat. No. 550878). We recommend purified mouse IgG1 κ monoclonal antibody MOPC-21 (Cat. No. 555746/554121) for immunofluorescent staining of human whole blood and Cat. No. 556648 for non-human primate cells.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
The MOPC-31C antibody has unknown specificity. The transplantable plasmacytoma MOPC-31C was induced by intraperitoneal injection of mineral oils into BALB/c mice. It was adapted to continuous cell culture by alternate passage in animals.
This antibody is routinely tested by flow cytometric analysis and by ELISA. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (2)
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Hay R, Caputo J, Chen TR, Macy M, McClintock P, Reid Y, ed. ATCC. Cell Lines and Hybridomas, Eighth Edition. 1994:75.
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Sibinovic KH, Potter M, Hoostelaere, Rode B, Wax J, ed. Catalogue of plasmacytomas and other tumors of the lymphoreticular system, 3rd edition. Kensington, Maryland: Litton Bionetics, Inc; 1976:1-33.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.