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      AKP Mouse Anti-Human IgG4
      Product Details
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      BD Pharmingen™
      Human (QC Testing)
      Mouse IgG1, κ
      ELISA (Routinely Tested)
      AB_647073
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with AKP under optimum conditions, and unconjugated antibody and free AKP were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Recommended Assay Procedures

      This antibody can be used to detect human IgG4. For the sandwich human IgG4 ELISA, AKP-conjugated JDC-14 is optimal as detecting MAb with G18-145 (Catalog No. 555784) as capture MAb. Researchers are encouraged to fully titrate each lot to obtain optimal results.  At high concentration, background may be observed on other Ig isotypes.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      5. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
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      555880 Rev. 9
      Antibody Details
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      JDC-14

      The JDC-14 antibody reacts specifically with human IgG4. It does not react with other Ig isotypes.

      555880 Rev. 9
      Format Details
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      AKP
      Alkaline phosphatase is an enzyme that is capable of cleaving various substrates, usually producing a detectable product. Antibodies, avidins, and other specific binding molecules conjugated with alkaline phosphatase, in conjunction with an appropriate substrate system, can be used for multiple ELISA or Immunohistochemical applications.
      AKP
      555880 Rev.9
      Citations & References
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      View product citations for antibody "555880" on CiteAb
      No Citations Found

      No Citations are available for this Product

      555880 Rev. 9

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.