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Recombinant Human β2 Microglobulin

Recombinant Human β2 Microglobulin

Product Details
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The major histocompatibility complex (MHC) gene locus encodes a group of highly polymorphic, cell surface proteins that play a broad role in the immune response to protein antigens. MHC molecules function by binding and presenting small antigenic protein fragments to antigen-specific receptors expressed by T cells (TCR). Class I MHC molecules consist of two separate polypeptide chains. The class I α chain is an MHC encoded, transmembrane polypeptide containing three extracellular domains: α1, α2 and α3. The second chain consists of a non-MHC encoded, 12 kD polypeptide called β2 microglobulin (β2M). Since β2M does not contain a transmembrane domain, it associates with the a chain through non-covalent interaction. This association is important for the stability of the MHC class I structure, its peptide-loading and its ability to present peptide antigen to CD8+ T cells. β2M is relatively invariant within each species. For example, human β2M is reported to have high affinity for human and mouse MHC class I heavy chains.

Human β2 Microglobulin recombinant protein was expressed in insect cells. The expressed protein was purified by a combination of ion-exchange and gel-filtration methods.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
551089 Rev. 1
Citations & References
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Development References (3)

  1. Parker KC, DiBrino M, Hull L, Coligan JE. The beta 2-microglobulin dissociation rate is an accurate measure of the stability of MHC class I heterotrimers and depends on which peptide is bound. J Immunol. 1992; 149(6):1896-1904. (Biology). View Reference
  2. Shields MJ, Moffat LE, Ribaudo RK. Functional comparison of bovine, murine, and human beta2-microglobulin: interactions with murine MHC I molecules. Mol Immunol. 1998; 35(14-15):919-928. (Biology). View Reference
  3. Silver ML, Parker KC, Wiley DC. Reconstitution by MHC-restricted peptides of HLA-A2 heavy chain with beta 2-microglobulin, in vitro. Nature. 1991; 350(6319):619-622. (Biology: Activation). View Reference
551089 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.