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Two-color flow cytometric analysis of TIM-4 expression on mouse resident peritoneal exudate cells. C57BL/6 mouse resident peritoneal exudate cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with PerCP-Cy™5.5 Rat Anti-Mouse CD11b (Cat. No. 550993/561114) and either PE Rat IgG2a, κ Isotype Control (Cat. No. 553930; Left Panel) or PE Rat Anti-Mouse TIM-4 antibody (Right Panel). Two-color flow cytometric contour plots showing the correlated expression patterns of TIM-4 (or Ig Isotype control staining) versus CD11b were derived from gated events with the forward and side light-scatter characteristics of viable resident peritoneal exudate cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
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BD Pharmingen™ PE Rat Anti-Mouse TIM-4
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Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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The RMT4-54 monoclonal antibody specifically binds to TIM-4. TIM-4 is encoded by Timd4 (T cell immunoglobulin and mucin domain containing 4). TIM-4 is also known as Spleen, mucin-containing, knockout of lymphotoxin protein (SMUCKLER). TIM-4 is a single-pass type I membrane transmembrane glycoprotein belonging to the TIM family of the immunoglobulin superfamily. TIM-4 is expressed by macrophages and at low levels by dendritic cells. TIM-4 is a phosphatidylserine receptor that enhances the phagocytosis of apoptotic cells. It can serve as a receptor for TIM-1, also known as the Hepatitis A virus cellular receptor 1 (Havcr1).

Development References (3)
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Curtiss ML, Gorman JV, Businga TR, et al. Tim-1 regulates Th2 responses in an airway hypersensitivity model. Eur J Immunol. 2012; 42(3):651-661. (Clone-specific: Flow cytometry). View Reference
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Freeman GJ, Casasnovas JM, Umetsu DT, DeKruyff RH. TIM genes: a family of cell surface phosphatidylserine receptors that regulate innate and adaptive immunity.. Immunol Rev. 2010; 235(1):172-89. (Biology). View Reference
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Nakayama M, Akiba H, Takeda K, et al. Tim-3 mediates phagocytosis of apoptotic cells and cross-presentation. Blood. 2009; 113(16):3821-3830. (Immunogen: Blocking, Flow cytometry, Functional assay, Inhibition, In vivo exacerbation). View Reference
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