The site is undergoing maintenance. Ordering can continue through fax and phone.
If you encounter problems while ordering. Please contact us at BDBCustomerService@bd.com
See More
BD Pharmingen™ PE Mouse Anti-Human CD272 (BTLA)
Clone J168-540 (RUO)
Two-color flow cytometric analysis of CD272 (BTLA) on human peripheral blood lymphocytes. Human whole blood was stained with FITC Mouse Anti-Human CD3 antibody(Cat. No. 555332/561806/561807) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Panel) or PE Mouse Anti-Human CD272 (BTLA) (Cat. No. 558485, Right Panel). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-color flow cytometric dot plots showing the correlated expression of CD3 versus CD272 (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD FACSCalibur™ System.
Two-color flow cytometric analysis of CD272 (BTLA) on human peripheral blood lymphocytes. Human whole blood was stained with FITC Mouse Anti-Human CD3 antibody(Cat. No. 555332/561806/561807) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Panel) or PE Mouse Anti-Human CD272 (BTLA) (Cat. No. 558485, Right Panel). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-color flow cytometric dot plots showing the correlated expression of CD3 versus CD272 (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD FACSCalibur™ System.
Two-color flow cytometric analysis of CD272 (BTLA) on human peripheral blood lymphocytes. Human whole blood was stained with FITC Mouse Anti-Human CD3 antibody(Cat. No. 555332/561806/561807) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Panel) or PE Mouse Anti-Human CD272 (BTLA) (Cat. No. 558485, Right Panel). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-color flow cytometric dot plots showing the correlated expression of CD3 versus CD272 (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD FACSCalibur™ System.
Two-color flow cytometric analysis of CD272 (BTLA) on human peripheral blood lymphocytes. Human whole blood was stained with FITC Mouse Anti-Human CD3 antibody(Cat. No. 555332/561806/561807) and either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Panel) or PE Mouse Anti-Human CD272 (BTLA) (Cat. No. 558485, Right Panel). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Two-color flow cytometric dot plots showing the correlated expression of CD3 versus CD272 (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD FACSCalibur™ System.
Product Details
BD Pharmingen™
BTLA1; B- and T-lymphocyte-associated protein
Human (QC Testing)
Mouse IgG1, κ
Human BTLA Recombinant Protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
IX 47
AB_647238
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
Lysing Solution 10X Concentrate IVD
Size
100 ML
Cat No.
349202
Lysing Buffer RUO
Size
100 ML
Cat No.
555899
PE Mouse IgG1, κ Isotype Control RUO
Size
0.1 mg
Cat No.
554680
FITC Mouse Anti-Human CD3 RUO
Size
25 Tests
Cat No.
561806
558485 Rev. 3
Antibody Details
J168-540
The J168-540 monoclonal antibody specifically binds to human CD272, also known as BTLA (B and T lymphocyte attenuator), an inhibitory receptor expressed in bone marrow and thymus on developing B and T cells. In the periphery, BTLA is expressed by B cells, T cells, bone marrow-derived dendritic cells, and macrophages. After T cell activation, BTLA appears to be expressed at higher levels on Th1 cell populations than in Th2 cell populations. Upon binding the herpesvirus entry mediator (HVEM/LIGHT-R/CD270), CD272 undergoes tyrosine phosphorylation and inhibits T cell proliferation in a BTLA-dependent manner. CD272 has structural similarities to two other lymphocyte inhibitory receptors, CTLA-4 and PD-1 and is a member of the CD28-like family of coreceptors. Based on these observations, CD272 is considered to be a negative regulator of lymphocyte activation and/or function.
558485 Rev. 3
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
496 nm, 566 nm
576 nm
558485 Rev.3
Citations & References
View product citations for antibody "558485" on CiteAb
Development References (5)
-
Compaan DM, Gonzalez LC, Tom I, Loyet KM, Eaton D, Hymowitz SG. Attenuating lymphocyte activity: the crystal structure of the BTLA-HVEM complex. J Biol Chem. 2005; 280(47):39553-39561. (Biology). View Reference
-
Hobo W, Norde WJ, Schaap N, et al. B and T lymphocyte attenuator mediates inhibition of tumor-reactive CD8+ T cells in patients after allogeneic stem cell transplantation. J Immunol. 2012; 189(1):39-49. (Clone-specific: Flow cytometry). View Reference
-
Llinas L, Lazaro A, de Salort J, Matesanz-Isabel J, Sintes J, Engel P. Expression profiles of novel cell surface molecules on B-cell subsets and plasma cells as analyzed by flow cytometry. Immunol Lett. 2011; 134(2):113-121. (Clone-specific: Flow cytometry). View Reference
-
Sedy JR, Gavrieli M, Potter KG, et al. B and T lymphocyte attenuator regulates T cell activation through interaction with herpesvirus entry mediator. Nat Immunol. 2005; 6(1):90-98. (Biology). View Reference
-
Watanabe N, Gavrieli M, Sedy JR, et al. BTLA is a lymphocyte inhibitory receptor with similarities to CTLA-4 and PD-1. Nat Immunol. 2003; 4(7):670-679. (Biology). View Reference
558485 Rev. 3
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.