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PE Mouse Anti-AhR
PE Mouse Anti-AhR

Multiparameter flow cytometric analysis of AhR expression

     Top Plots - AhR expression in human peripheral blood mononuclear cells. PBMCs were fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat No. 562574/562725) and stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Plot) or PE Mouse Anti-AhR antibody (Cat. No. 565711; Right Plot).

     Bottom Plots - AhR expression in mouse splenic leucocytes. Mouse splenic leucocytes were similarly fixed, permeabilized, stained, and analyzed.

     Two-parameter flow cytometric contour plots showing the correlated expression of AhR (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.

Multiparameter flow cytometric analysis of AhR expression

     Top Plots - AhR expression in human peripheral blood mononuclear cells. PBMCs were fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat No. 562574/562725) and stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Plot) or PE Mouse Anti-AhR antibody (Cat. No. 565711; Right Plot).

     Bottom Plots - AhR expression in mouse splenic leucocytes. Mouse splenic leucocytes were similarly fixed, permeabilized, stained, and analyzed.

     Two-parameter flow cytometric contour plots showing the correlated expression of AhR (or Ig Isotype control staining) versus side light-scatter (SSC-A) signals were derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.

Product Details
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BD Pharmingen™
AHR; Aryl hydrocarbon R; Ah-Receptor; Aromatic hydrocarbon receptor; bHLHe7
Human (QC Testing), Mouse (Tested in Development)
Mouse IgG1, κ
Human AhR Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
AB_2739336
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. An isotype control should be used at the same concentration as the antibody of interest.
565711 Rev. 1
Antibody Details
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T49-550

The T49-550 monoclonal antibody recognizes the human Aryl Hydrocarbon Receptor (AhR), which is also known as Aromatic Hydrocarbon Receptor, and cross-reacts with the mouse AhR. AhR is a member of basic helix-loop-helix transcription factors (Class E basic helix-loop-helix protein 76, bHLHe76). AhR can bind to a variety of endogenous and exogenous ligands including tryptophan metabolites, bacterial pigments, plant flavonoids, synthetic polycyclic aromatic hydrocarbons, or dioxin-like compounds. Upon ligand binding, cytoplasmic AhR undergoes conformational changes and translocates into the nucleus to induce gene transcription. AhR is widely expressed in mammalian tissues with the highest expression levels in the liver and lung. A number of studies suggest that AhR regulates inflammation mainly by attenuating the production of inflammatory cytokines by dendritic cells and macrophages.  In addition, AhR can regulate the expansion or effector functions of Th17 cells, IL-10-secreting regulatory T (Tr1) cells, and subsets of intraepithelial lymphocytes (IEL) and innate lymphoid cells (ILC), keratinocytes, and Langerhans cells.

        

565711 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
565711 Rev.1
Citations & References
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Development References (8)

  1. Denison MS, Nagy SR. Activation of the aryl hydrocarbon receptor by structurally diverse exogenous and endogenous chemicals. Annu Rev Pharmacol Toxicol. 2003; 43(309):334. (Biology). View Reference
  2. Gonzalez FJ, Fernandez-Salguero P. The aryl hydrocarbon receptor: studies using the AHR-null mice. Drug Metab Dispos. 1998; 26(12):1194-1198. (Biology). View Reference
  3. Henry EC, Bemis JC, Henry O, Kende AS, Gasiewicz TA. A potential endogenous ligand for the aryl hydrocarbon receptor has potent agonist activity in vitro and in vivo. Arch Biochem Biophys. 2006; 450(1):67-77. (Biology). View Reference
  4. Ho PP, Steinman L. The aryl hydrocarbon receptor: a regulator of Th17 and Treg cell development in disease. Cell Res. 2008; 18(6):605-608. (Biology). View Reference
  5. Ramsay G, Cantrell D. Environmental and metabolic sensors that control T cell biology. Front Immunol. 2015; 6(1):8. (Biology). View Reference
  6. Simones T, Shepherd DM. Consequences of AhR activation in steady-state dendritic cells. Toxicology. 2011; 119(2):293-307. (Biology). View Reference
  7. Stockinger B, Di Meglio P, Gialitakis M, Duarte JH. The aryl hydrocarbon receptor: multitasking in the immune system. Annu Rev Immunol. 2014; 32(403):432. (Biology). View Reference
  8. Veldhoen M, Hirota K, Christensen J, O'Garra A, Stockinger B. Natural agonists for aryl hydrocarbon receptor in culture medium are essential for optimal differentiation of Th17 T cells. J Exp Med. 2009; 206(1):43-49. (Biology). View Reference
View All (8) View Less
565711 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.