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Fluorescent Cell Barcoding Wash Buffer

Fluorescent Cell Barcoding Wash Buffer

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Description

BD Phosflow™ Fluorescent Cell Barcoding Wash Buffer can be used for washing and staining cells after Fluorescent Cell Barcoding, ie, labeling cells with Cell Barcoding Dyes (either CBD450 or CBD500 dye or mixtures of both). Moreover, the Fluorescent Cell Barcoding Wash Buffer is useful for the dilution and application of fluorescent antibodies to barcoded cells as well as for the suspension, washing, and short-term storage of barcoded cells destined for flow cytometric analysis. Fluorescent Cell Barcoding Wash Buffer is formulated as a neutral pH (pH 7.4)-buffered salt solution that is supplemented with fetal bovine serum and bovine serum albumin. As such, Fluorescent Cell Barcoding Wash Buffer is designed to maintain single-cell suspensions well and to maximize fluorescence signal intensities. The Fluorescent Cell Barcoding Wash Buffer contains the metabolic inhibitor, sodium azide as a preservative.



Recommended Assay Procedures

Dilute BD Phosflow™ Fluorescent Cell Barcoding Wash Buffer 1:4 in 1× Phosphate Buffered Saline (PBS) (ie, 1 volume of Wash Buffer plus 3 parts PBS).

For detailed instructions on the use of the BD Phosflow™ Fluorescent Cell Barcoding Wash Buffer, refer to the Technical Data Sheet for the BD Phosflow™  Violet Fluorescent Cell Barcoding Dyes (Cat. No. 561570). Please see the Suggested Companion Products section of this Technical Data Sheet.

Product Notices

  1. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Patent Pending.
  4. Reagents which contain preservatives may be toxic if ingested, inhaled, or in contact with skin.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
561550 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.