Flow cytometric analysis of CD59 expression on human glycosylphosphatidiylinositol (GPI) anchor -defective cell line (left panel) or K562 cells expressing GPI anchor protein (right panel). Whole blood was stained with either FITC Mouse IgG2a, κ Isotype Control (Cat. No. 555573; dashed line histograms) or FITC Mouse Anti-Human CD59 antibody (Cat. No. 555763/560954; solid line histograms). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). Fluorescent histograms depicting CD59 (or Ig isotype control) staining were derived from gated events with the forward and side-light scatter characteristics of K562 cells. Flow cytometric analysis was performed using a BD FACScan™ Cytometer System.
Flow cytometric analysis of CD59 expression on human glycosylphosphatidiylinositol (GPI) anchor -defective cell line (left panel) or K562 cells expressing GPI anchor protein (right panel). Whole blood was stained with either FITC Mouse IgG2a, κ Isotype Control (Cat. No. 555573; dashed line histograms) or FITC Mouse Anti-Human CD59 antibody (Cat. No. 555763/560954; solid line histograms). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). Fluorescent histograms depicting CD59 (or Ig isotype control) staining were derived from gated events with the forward and side-light scatter characteristics of K562 cells. Flow cytometric analysis was performed using a BD FACScan™ Cytometer System.
Product Details
BD Pharmingen™
HRF-20; MAC-inhibitory protein; MAC-IP; MACIF; MEM43; MIRL; Protectin; 1F5
Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)
Mouse IgG2a, κ
Human Erythrocytes
Flow cytometry (Routinely Tested)
20 µl
V S006
966
AB_396103
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Lysing Solution 10X Concentrate IVD
Size
100 mL
Cat No.
349202
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
FITC Mouse IgG2a, κ Isotype Control RUO
Size
100 Tests
Cat No.
555573
FITC Mouse Anti-Human CD59 RUO
Size
25 Tests
Cat No.
560954
555763 Rev. 9
Antibody Details
p282 (H19)
The p282 (HI9) monoclonal antibody specifically binds to CD59, a 19 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein, expressed on hematopoietic and non-hematopoietic cells. Because of its interaction with complement activated products, CD59 has been termed membrane-attack-complex-inhibitory factor (MACIF), homologus restriction factor (HRF20), membrane inhibitor of reactive lysis (MIRL) and Protectin. It inhibits the cytolytic activity of the complement system by binding to C8 and C9, thereby blocking the assembly of the membrane attack complex. CD59 also participates in spontaneous T-cell/erythrocyte adhesion, interacts with CD2, and plays a role in T-cell activation.
555763 Rev. 9
Format Details
FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
FITC
Blue 488 nm
494 nm
518 nm
555763 Rev.9
Citations & References
View product citations for antibody "555763" on CiteAb
Development References (7)
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Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
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Davies A, Lachmann PJ. Membrane defence against complement lysis: the structure and biological properties of CD59. Immunol Res. 1993; 12(3):258-275. (Biology). View Reference
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Deckert M, Kubar J, Bernard A. CD58 and CD59 molecules exhibit potentializing effects in T cell adhesion and activation. J Immunol. 1992; 148(3):672-677. (Biology). View Reference
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Deckert M, Kubar J, Zoccola D, et al. CD59 molecule: a second ligand for CD2 in T cell adhesion. Eur J Immunol. 1992; 22(11):2943-2947. (Biology). View Reference
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Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
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Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
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Whitlow MB, Iida K, Stefanova I, Bernard A, Nussenzweig V. H19, a surface membrane molecule involved in T-cell activation, inhibits channel formation by human complement. Cell Immunol. 1990; 126(1):176-184. (Biology). View Reference
555763 Rev. 9
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.