The 837535 monoclonal antibody specifically recognizes Sialic acid-binding Ig-like lectin 8 (Siglec-8) which is also known as Sialoadhesin family member 2 (SAF-2). Siglec-8 is a single-pass, type I transmembrane glycoprotein that is encoded by SIGLEC8 which belongs to the Sialic acid-binding Ig-like lectins (Siglecs) superfamily. Siglec-8 has an Ig-like V-set domain followed by two Ig-like C-type domains in its extracellular region. Its intracellular region contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an immunoreceptor tyrosine-based switch motif (ITSM) which might contribute to inhibitory signal transduction upon engagement of this receptor. A short form of Siglec-8 that results from alternative splicing and which lacks most of the intracellular domain has also been identified. Siglec-8 is expressed on eosinophils and mast cells, and weakly on basophils. Siglec-8 appears to serve as an adhesion molecule which mediates cell-cell interactions through its binding to 6' sulfated sialyl Lewis X carbohydrate structures. Siglec-8 seems to be involved in signal transduction since engagement of Siglec-8 can induce apoptosis in eosinophils or inhibit mast cell degranulation.
This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.