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BV510 Mouse Anti-Rat CD6

BD OptiBuild™ BV510 Mouse Anti-Rat CD6

Name: BV510 Mouse Anti-Rat CD6
Brand: BV510 Mouse Anti-Rat CD6
SKU: 745045

Clone OX-52 (RUO)

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Product Details

Brand: BD OptiBuild™

Alternative Name: OX-52 Antigen; Pan-T Cells; Cd6; Tp120

Reactivity: Rat (Tested in Development)

Isotype: Mouse BALB/c IgG2a, κ

Immunogen: PVG-RT1c rat thoracic-duct leukocytes and splenocytes

Application: Flow cytometry (Qualified)

Concentration: 0.2 mg/ml

RRID: AB_2742670

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV510 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

This antibody was developed for use in flow cytometry.
The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
Researchers should determine the optimal concentration of this reagent for their individual applications.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
BD Horizon Brilliant Violet 510 is covered by one or more of the following US patents: 8,575,303; 8,354,239.

Companion Products

Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) RUO

Size 0.1 mg Cat No. 553141

Stain Buffer (FBS) RUO

Size 500 ML Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 ML Cat No. 554657

Brilliant Stain Buffer RUO

Size 100 Tests Cat No. 563794

Lysing Buffer RUO

Size 100 ML Cat No. 555899

BV510 Mouse IgG2a, κ Isotype Control RUO

Size 50 µg Cat No. 563027

View Details

745045 Rev. 1

Antibody Details

OX-52

The OX-52 monoclonal antibody specifically recognizes CD6, a 95-120–kDa antigen expressed on T lymphocytes and thymocytes expressing αβ and γδ Tcell receptors. Rat NK cells express low levels of CD6 and are CD8a+, CD8b-, CD5-, and CD3-. In immunoprecipitation experiments, CD6 has been shown to co-precipitate with CD5 and to play a key role in CD5 tyrosine phosphorylation.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50-nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser.

745045 Rev. 1

Format Details

BV510

The BD Horizon Brilliant Violet™ 510 (BV510) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye with an excitation maximum (Ex Max) at 327-nm / 405-nm and an emission maximum (Em Max) at 512-nm. BV510, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 510-nm (e.g., a 525/50 bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: BV510

Excitation Max: 327 nm, 405 nm

Emission Max: 512 nm

745045 Rev.1

Citations & References

View product citations for antibody "745045" on CiteAb

Development References (5)

Brunson ME, Tchervenkov JI, Alexander JW, Cofer BR. Partial T-cell depletion with monoclonal antibody improves the enhancing effect of donor-specific transfusion plus cyclosporine. Transplant Proc. 1991; 23(1):307-308. (Clone-specific: Depletion). View Reference
Castro MA, Nunes RJ, Oliveira MI. OX52 is the rat homologue of CD6: evidence for an effector function in the regulation of CD5 phosphorylation. J Leukoc Biol. 2003; 73(1):183-190. (Clone-specific: Immunoprecipitation, Stimulation). View Reference
Lawetzky A, Tiefenthaler G, Kubo R, Hunig T. Identification and characterization of rat T cell subpopulations expressing T cell receptors alpha/beta and gamma/delta. Eur J Immunol. 1990; 20(2):343-349. (Clone-specific: Immunoprecipitation). View Reference
Robinson AP, Puklavec M, Mason DW. MRC OX-52: a rat T-cell antigen. Immunology. 1986; 57(4):527-531. (Immunogen: Immunoprecipitation). View Reference
Vaage JT, Dissen E, Ager A, Fossum S, Rolstad B. Allospecific recognition of hemic cells in vitro by natural killer cells from athymic rats: evidence that allodeterminants coded for by single major histocompatibility complex haplotypes are recognized. Eur J Immunol. 1991; 21(9):2167-2175. (Biology). View Reference

View All (5)

745045 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.