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BV421 Mouse Anti-Human Desmoglein 2
BV421 Mouse Anti-Human Desmoglein 2
Flow cytometric analysis of Desmoglein 2 expression on viable Human CD34+ lymphoid cells. Human peripheral blood mononuclear cells (PBMC) were stained with APC Mouse Anti-Human CD34 antibody (Cat. No. 555824/560940) and with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; dashed line histogram) or BD Horizon™ BV421 Mouse Anti-Human Desmoglein 2 antibody (Cat. No. 569349/569350; solid line histogram). BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The fluorescence histogram showing Desmoglein 2 expression (or Ig Isotype control staining) [Right Plot] was derived from gated CD34 positive cells [Left Plot as indicated] with the forward and side light-scatter characteristics of viable (7-AAD-negative) lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software.
BV421 Mouse Anti-Human Desmoglein 2
Flow cytometric analysis of Desmoglein 2 expression on viable Human HeLa cells. Cells from the HeLa (Cervical adenocarcinoma, ATCC® CCL-2™) cell line were stained with BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; dashed line histogram) or with BD Horizon™ BV421 Mouse Anti-Human Desmoglein 2 antibody (Cat. No. 569349/569350; solid line histogram). BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis.  The fluorescence histogram showing the expression of Human Desmoglein 2 (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software.
Flow cytometric analysis of Desmoglein 2 expression on viable Human CD34+ lymphoid cells. Human peripheral blood mononuclear cells (PBMC) were stained with APC Mouse Anti-Human CD34 antibody (Cat. No. 555824/560940) and with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; dashed line histogram) or BD Horizon™ BV421 Mouse Anti-Human Desmoglein 2 antibody (Cat. No. 569349/569350; solid line histogram). BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The fluorescence histogram showing Desmoglein 2 expression (or Ig Isotype control staining) [Right Plot] was derived from gated CD34 positive cells [Left Plot as indicated] with the forward and side light-scatter characteristics of viable (7-AAD-negative) lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software.
Flow cytometric analysis of Desmoglein 2 expression on viable Human HeLa cells. Cells from the HeLa (Cervical adenocarcinoma, ATCC® CCL-2™) cell line were stained with BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; dashed line histogram) or with BD Horizon™ BV421 Mouse Anti-Human Desmoglein 2 antibody (Cat. No. 569349/569350; solid line histogram). BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis.  The fluorescence histogram showing the expression of Human Desmoglein 2 (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software.
Product Details
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BD Horizon™
DSG2; CDHF5; Cadherin family member 5; Desmoglein-2; desmoglein-2
Human (QC Testing)
Mouse IgG1, κ
Human Full-length recomb. Desmoglein 2
Flow cytometry (Routinely Tested)
5 µl/test
1829
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

   BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.  However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

   For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant™ Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant™ Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant™ Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant™ Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant™ Stain Buffer Plus (Cat. No. 566385).

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
  7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  8. For U.S. patents that may apply, see bd.com/patents.
569350 Rev. 3
Antibody Details
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6D8

The 6D8 monoclonal antibody specifically recognizes the extracellular region of human Desmoglein 2 which is also known as Cadherin family member 5 (CDHF5). Desmoglein 2 is a type 1 transmembrane glycoprotein encoded by DSG2 which belongs to the cadherin cell adhesion molecule superfamily. Desmoglein 2 is variably expressed by epithelial cells, cardiomyocytes, endothelial progenitors associated with neoangiogenesis, and by CD34+CD45lo hematopoietic stem and progenitor cells. Desmoglein-2 plays essential roles in cellular adhesion, migration, proliferation, apoptosis and intracellular signaling. This calcium-dependent adhesion molecule is an essential component of  intercellular desmosome junctions. These adhesive desmosomal junctions serve to link adjacent cells together such as in the formation of the intestinal epithelial barrier. The desmosomes can also connect with the cytoskeleton through interactions with cytoplasmic plaque proteins and intermediate filaments. Desmoglein 2 can also serve as a receptor for adenoviruses.

569350 Rev. 3
Format Details
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV421
Violet 405 nm
407 nm
423 nm
569350 Rev.3
Citations & References
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Development References (5)

  1. Ebert LM, Tan LY, Johan MZ, et al. A non-canonical role for desmoglein-2 in endothelial cells: implications for neoangiogenesis.. Angiogenesis. 2016; 19(4):463-86. (Clone-specific: Flow cytometry). View Reference
  2. Ebert LM, Vandyke K, Johan MZ, et al. Desmoglein-2 expression is an independent predictor of poor prognosis patients with multiple myeloma.. Mol Oncol. 2022; 16(6):1221-1240. (Clone-specific: Flow cytometry). View Reference
  3. Tan LY, Mintoff C, Johan MZ, et al. Desmoglein 2 promotes vasculogenic mimicry in melanoma and is associated with poor clinical outcome.. Oncotarget. 2016; 7(29):46492-46508. (Clone-specific: Flow cytometry). View Reference
  4. Wahl JK, Sacco PA, McGranahan-Sadler TM, Sauppé LM, Wheelock MJ, Johnson KR. Plakoglobin domains that define its association with the desmosomal cadherins and the classical cadherins: identification of unique and shared domains.. J Cell Sci. 1996; 109 ( Pt 5):1143-54. (Immunogen: Cell differentiation, Immunoprecipitation, Radioimmunoassay). View Reference
  5. Wang H, Li ZY, Liu Y, et al. Desmoglein 2 is a receptor for adenovirus serotypes 3, 7, 11 and 14.. Nat Med. 2011; 17(1):96-104. (Clone-specific: Flow cytometry, Immunofluorescence). View Reference
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569350 Rev. 3

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.