The VIM15 monoclonal antibody specifically binds to CD92 which is also known as Solute carrier family 44 member 1 (SLC44A1), or Choline transporter-like protein 1 (CHTL1). CD92 is a multipass membrane glycoprotein that belongs to the choline transporter-like family. It contains a tyrosine-based inhibitory motif (ITIM) in its cytoplasmic C-terminus. CD92 appears to be involved in choline transport for cell membrane phospholipid synthesis and in regulatory cell-signaling pathways. CD92 is strongly expressed by monocytes and at lower levels by granulocytes and some dendritic cells. It is generally expressed at low levels by B cells, endothelial cells, epithelial cells, and subsets of T cells, or NK cells. The VIM15 antibody has been shown to augment lipopolysaccharide-induced IL-10 production by monocyte-derived dendritic cells (Mo-DC). When cultured with ionomycin or calcium ionophore, maturing Mo-DC showed downregulation of CD92 expression.
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.