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BUV563 Mouse Anti-Human FCRL5 (CD307e)
Product Details
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BD OptiBuild™
BXMAS1; FCRH5; Fc receptor-like protein 5; IRTA2; PRO820; fc receptor homolog 5; fcR-like protein 5
Human (Tested in Development)
Mouse BALB/c IgG2a, κ
Human FCRL5 Transfected Cell Line
Flow cytometry (Qualified)
0.2 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV563 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at
  7. Please refer to for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
749598 Rev. 2
Antibody Details
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The 509F6 monoclonal antibody specifically binds to Fc receptor-like 5 (FCLR5), a type I transmembrane glycoprotein that is also known as CD307e, FCRH5 (Fc receptor homolog 5) and IRTA2 (immunoglobulin superfamily receptor translocation associated 2). FCRL5 (CD307e) is a member of the Immunoglobulin (Ig) gene superfamily with 9 extracellular Ig domains, an intracellular immunoreceptor tyrosine-based activating motif (ITAM)-like sequence and two intracellular immunoreceptor tyrosine-based inhibitory motif (ITIM)-like sequences. Like other Fc receptor-like proteins, FCRL5 (CD307e) is homologous to FcγR1 (CD64) and binds to immunoglobulins, specifically the IgG isotypes, and it may play a role in the regulatory interactions of immune complexes with B lymphocytes. FCRL5 (CD307e) is differentially expressed in the B cell lineage, with little expression on peripheral blood cells and upregulated expression on plasma cells and some leukemias and lymphomas. mAb 509F6 binds to an epitope within the first 3 Ig domains of FCRL5 and blocks FCRL5's interaction with immune complexes.

The antibody was conjugated to BD Horizon™ BUV563 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 which has an Ex Max of 348 nm and an acceptor dye. The tandem has an Em Max at 563 nm. BD Horizon BUV563 can be excited by the 355 nm ultraviolet laser. On instruments with a 561 nm Yellow-Green laser, the recommended bandpass filter is 585/15 nm with a 535 nm long pass to minimize laser light leakage. When BD Horizon BUV563 is used with an instrument that does not have a 561 nm laser, a 560/40 nm filter with a 535 nm long pass may be more optimal. Due to the excitation and emission characteristics of the acceptor dye, there may be spillover into the PE and PE-CF594 detectors. However, the spillover can be corrected through compensation as with any other dye combination.

749598 Rev. 2
Format Details
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The BD Horizon Brilliant™ Ultraviolet 563 (BUV563) Dye is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This tandem fluorochrome is comprised of a BUV395 donor with an excitation maximum (Ex Max) of 350-nm and an acceptor dye with an emission maximum (Em Max) at 564-nm. BUV563, driven by BD innovation, is designed to be excited by the ultraviolet laser (355-nm) and detected using an optical filter centered near 560-nm (e.g., a 560/40 or a 585/15-nm bandpass filter). The acceptor dye can be excited by the Blue (488-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Ultraviolet 355 nm
350 nm
564 nm
749598 Rev.2
Citations & References
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Development References (5)

  1. Damdinsuren B, Dement-Brown J, Li H, Tolnay M. B cell receptor induced Fc receptor-like 5 expression is mediated by multiple signaling pathways converging on NF-κB and NFAT. Mol Immunol. 2016; 73:112-21. (Clone-specific). View Reference
  2. Li FJ, Won WJ, Becker EJ, et al. Emerging roles for the FCRL family members in lymphocyte biology and disease.. Curr Top Microbiol Immunol. 2014; 382:29-50. (Biology). View Reference
  3. Li H, Borrego F, Nagata S, Tolnay M. Fc Receptor-like 5 Expression Distinguishes Two Distinct Subsets of Human Circulating Tissue-like Memory B Cells. J Immunol. 2016; 196(10):4064-74. (Clone-specific). View Reference
  4. Polson AG, Zheng B, Elkins K, et al. Expression pattern of the human FcRH/IRTA receptors in normal tissue and in B-chronic lymphocytic leukemia. Int Immunol. 2006; 18(9):1363-1373. (Biology). View Reference
  5. Wilson TJ, Fuchs A, Colonna M. Cutting edge: human FcRL4 and FcRL5 are receptors for IgA and IgG. J Immunol. 2012; 188(10):4741-4745. (Immunogen). View Reference
View All (5) View Less
749598 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.