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APC Mouse Anti-Human CD11A
APC Mouse Anti-Human CD11A

Flow cytometric analysis of CD11a expression on human peripheral blood lymphocytes. Whole blood was stained with either APC Mouse Anti-Human CD11a (Cat. No. 550852/559875; solid line histogram) or APC Mouse IgG1, κ Isotype Control (Cat. No. 550751; dashed line histogram). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes. Flow cytometric analysis was performed on a BD FACScan™ system.

Flow cytometric analysis of CD11a expression on human peripheral blood lymphocytes. Whole blood was stained with either APC Mouse Anti-Human CD11a (Cat. No. 550852/559875; solid line histogram) or APC Mouse IgG1, κ Isotype Control (Cat. No. 550751; dashed line histogram). Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable lymphocytes. Flow cytometric analysis was performed on a BD FACScan™ system.

Product Details
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BD Pharmingen™
LFA-1α; Lymphocyte (Leukocyte) function-associated antigen 1 α chain; ITGAL
Human (QC Testing), Rhesus, Cynomolgus, Baboon, Dog, Rabbit (Tested in Development)
Mouse IgG1, κ
Flow cytometry (Routinely Tested)
20 µl
IV N231
3683
AB_2129113
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
  7. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
  8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
559875 Rev. 7
Antibody Details
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HI111

The HI111 monoclonal antibody specifically binds to CD11a, the 180 kDa integrin α chain. This type I transmembrane glycoprotein associates with CD18 (integrin β2) to form the heterodimeric glycoprotein CD11a/CD18. This heterodimer is also known as the lymphocyte (leukocytes) function associated antigen-1 (LFA-1) that is expressed on all leukocytes. LFA-1 is an adhesion molecule involved in lymphocyte and granulocyte functions. LFA-1 mediates adhesion of lymphoid cells to the vascular endothelium in association with its ligand, and the intracellular adhesion molecule-1 (ICAM-1), CD54. Other ligands are ICAM-2 (CD102) and ICAM-3 (CD50).

Clone HI111 also cross reacts with all leukocytes of baboon, and both rhesus and cynomolgus macaque monkeys. The distribution of leukocytes is similar to that observed with peripheral blood leukocytes from normal human donors, with the lymphocyte population also showing a bimodal staining pattern.

559875 Rev. 7
Format Details
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APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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APC
Red 627-640 nm
651 nm
660 nm
559875 Rev.7
Citations & References
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Development References (4)

  1. Dustin ML, Springer TA. Lymphocyte function-associated antigen-1 (LFA-1) interaction with intercellular adhesion molecule-1 (ICAM-1) is one of at least three mechanisms for lymphocyte adhesion to cultured endothelial cells. J Cell Biol. 1988; 107(1):321-331. (Biology). View Reference
  2. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
  3. Lin G-X, Yang X, Hollemweguer E, et al. Cross-reactivity of CD antibodies in eight animal species. In: Mason D. David Mason .. et al., ed. Leucocyte typing VII : white cell differentiation antigens : proceedings of the Seventh International Workshop and Conference held in Harrogate, United Kingdom. Oxford: Oxford University Press; 2002:519-523.
  4. Rothlein R, Dustin ML, Marlin SD, Springer TA. A human intercellular adhesion molecule (ICAM-1) distinct from LFA-1. J Immunol. 1986; 137(4):1270-1274. (Biology). View Reference
View All (4) View Less
559875 Rev. 7

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.