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Alexa Fluor® 647 Mouse Anti-p38 MAPK (pT180/pY182)

BD Phosflow™ Alexa Fluor® 647 Mouse Anti-p38 MAPK (pT180/pY182)

Clone 36/p38 (pT180/pY182) (RUO)

612595
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EA (1 Each)
50 Tests
Product Details
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BD Phosflow™
MAPK14; p38 MAP kinase; p38 MAPK; RK; CSBP2
Human (QC Testing), Mouse, Rat (Tested in Development)
Mouse IgG1, κ
Phosphorylated Human p38 MAPK (pT180/pY182) Peptide
Intracellular staining (flow cytometry) (Routinely Tested)
20 µl
AB_399878
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Recommended Assay Procedures

Investigators are encouraged to reference http://www.bdbiosciences.com/research/ics/resources/index.jsp for more information concerning BD Phosflow™ protocols and other resources.

Data Sheets

612595 Rev. 11
Antibody Details
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36/p38 (pT180/pY182)

Activation of the immune and inflammatory responses often involves the recognition of bacterial endotoxin (lipopolysaccharide or LPS).  Binding of LPS by monocytes results in the production and release of proinflammatory cytokines, such as IL-1 and TNF.  LPS-induced signaling cascades involve members of the Ser/Thr protein kinase family known as the Mitogen Activated Protein Kinases (MAPKs).  MAPK signal transduction pathways mediate the effects of various extracellular stimuli on biological processes such as proliferation, differentiation, and death.  The p38 MAPKs include p38α (MAPK14), β (MAPK11), γ (MAPK12), and δ (MAPK13).  These Ser/Thr kinases are activated by dual phosphorylation on threonine (T) and tyrosine (Y) within the motif Thr-Gly-Tyr located in kinase subdomain VIII.  Activation of p38 MAPK is mediated specifically by the MAP Kinase Kinases, MKK3, MKK4, and MKK6.  This leads to the activation of multiple transcription factors (NF-κB, ATF-2, Elk-1, and CHOP) that induce expression of many different genes, including proinflammatory cytokine genes.  Thus, p38 MAPKs are central kinases in multiple signal transduction pathways.

The 36/p38 (pT180/pY182) monoclonal antibody recognizes the conserved dual phosphorylated site pT180/pY182 of p38α, β, γ, and δ.

612595 Rev. 11
Format Details
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Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
653 nm
669 nm
612595 Rev.11
Citations & References
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No Citations Found

No Citations Are Available for this Product

612595 Rev. 11

 

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.