Skip to main content Skip to navigation
Blank Calibration Particles, 6.0 - 6.4 µm

Sphero™ Blank Calibration Particles, 6.0 - 6.4 µm

Blank Calibration Particles, 6.0 - 6.4 µm

Rainbow Particle peak separation based on FL1 fluorescence intensity expressed in relative channel numbers. The Blank Calibration Particles correspond to peak 1 in the Rainbow Calibration Particles depicted in the figure.

633753
Sign In
EA (1 Each)
5 mL
Product Details
Down Arrow

Product Details

Description

The vial contains a single population of Blank Calibration Particles that are dyed to a single fluorescent intensity. The intensity of this particle corresponds to the dimmest intensity. The intensity of this particle corresponds to the dimmest intensity peak in the Rainbow Calibration Particles, 6.0 - 6.4 µm (Cat. No. 556288). Every Rainbow Particle contains a mixture of fluorophores that are excited at any wavelength from365 - 650 nm. The Rainbow Particles have emission spectra compatible with many common fluorophores used for immunofluorescent staining with flow cytometric analysis.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

This particle mixture (~10x10^6 particles/ml) is useful for routine calibration of flow cytometers. Before use, resuspend the particles by vortexing. Dilution of 3 - 5 drops of particles to 1 ml of sheath fluid will provide an adequate number of particles for flow cytometric analysis.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).

Required Companion Products

cDNA Kit
Product Image
Size:1 Each
Status:RUO
Cat No.:633773
Your Price:Sign In
cart iconAdd To Cart
Targeted mRNA and AbSeq Amplification Kit
Product Image
Size:1 Each
Status:RUO
Cat No.:633774
Your Price:Sign In
cart iconAdd To Cart
Cartridge Kit
Product Image
Size:1 Each
Status:RUO
Cat No.:633733
Your Price:Sign In
cart iconAdd To Cart

Data Sheets

556296 Rev. 5

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.