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      PE Rat Anti-Mouse CD127
      552543Image1.png

      Expression of CD127 (IL-7Rá) in the bone marrow. BALB/c bone marrow leukocytes were stained with either PE-conjugated rat IgG2b, κ isotype control mAb A95-1 (Cat. No. 553989, left panel) or PE-conjugated  mAb SB/199 (right panel) and a cocktail of FITC-conjugated antibodies to the lineage markers CD3e (mAb 145-2C11, Cat. No. 553061/553062), CD8a (mAb 53-6.7, Cat. No. 553030/553031), CD11b (mAb M1/70, Cat. No. 557396/553310), CD19 (mAb 1D3, Cat. No. 557398/553785), CD45R/B220 (mAb RA3- 6B2, Cat. No. 553087/553088), and Ly-6G and Ly-6C (mAb RB6-8C5, Cat.  No. 553126/553127). Flow cytometry was performed on a BD FACSCalibur™ Flow  Cytometry System.

      552543_image2.png

      Expression of CD127 (IL-7Rá) in the bone marrow. BALB/c bone marrow leukocytes were stained with either PE-conjugated rat IgG2b, κ isotype control mAb A95-1 (Cat. No. 553989, left panel) or PE-conjugated  mAb SB/199 (right panel) and a cocktail of FITC-conjugated antibodies to the lineage markers CD3e (mAb 145-2C11, Cat. No. 553061/553062), CD8a (mAb 53-6.7, Cat. No. 553030/553031), CD11b (mAb M1/70, Cat. No. 557396/553310), CD19 (mAb 1D3, Cat. No. 557398/553785), CD45R/B220 (mAb RA3- 6B2, Cat. No. 553087/553088), and Ly-6G and Ly-6C (mAb RB6-8C5, Cat.  No. 553126/553127). Flow cytometry was performed on a BD FACSCalibur™ Flow  Cytometry System.

      552543Image1.png 552543_image2.png Red-Cap-Blue-Label.jpg

      Expression of CD127 (IL-7Rá) in the bone marrow. BALB/c bone marrow leukocytes were stained with either PE-conjugated rat IgG2b, κ isotype control mAb A95-1 (Cat. No. 553989, left panel) or PE-conjugated  mAb SB/199 (right panel) and a cocktail of FITC-conjugated antibodies to the lineage markers CD3e (mAb 145-2C11, Cat. No. 553061/553062), CD8a (mAb 53-6.7, Cat. No. 553030/553031), CD11b (mAb M1/70, Cat. No. 557396/553310), CD19 (mAb 1D3, Cat. No. 557398/553785), CD45R/B220 (mAb RA3- 6B2, Cat. No. 553087/553088), and Ly-6G and Ly-6C (mAb RB6-8C5, Cat.  No. 553126/553127). Flow cytometry was performed on a BD FACSCalibur™ Flow  Cytometry System.

      Expression of CD127 (IL-7Rá) in the bone marrow. BALB/c bone marrow leukocytes were stained with either PE-conjugated rat IgG2b, κ isotype control mAb A95-1 (Cat. No. 553989, left panel) or PE-conjugated  mAb SB/199 (right panel) and a cocktail of FITC-conjugated antibodies to the lineage markers CD3e (mAb 145-2C11, Cat. No. 553061/553062), CD8a (mAb 53-6.7, Cat. No. 553030/553031), CD11b (mAb M1/70, Cat. No. 557396/553310), CD19 (mAb 1D3, Cat. No. 557398/553785), CD45R/B220 (mAb RA3- 6B2, Cat. No. 553087/553088), and Ly-6G and Ly-6C (mAb RB6-8C5, Cat.  No. 553126/553127). Flow cytometry was performed on a BD FACSCalibur™ Flow  Cytometry System.

      Product Details
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      BD Pharmingen™
      Interleukin-7 receptor alpha chain; IL-7R alpha; IL-7RA; IL-7Rα; Il7r
      Mouse (QC Testing)
      Rat IgG2b, κ
      BALB/c mouse pre-B cell line 1A9
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      AB_394417
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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      Recommended Assay Procedures

      Note: Investigators are highly encouraged to titrate the antibody up to saturating levels for optimal performance, minimizing the risk for dim staining.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. An isotype control should be used at the same concentration as the antibody of interest.
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      552543 Rev. 2
      Antibody Details
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      SB/199

      The SB/199 monoclonal antibody specifically binds to mouse CD127 which is also known as, the Interleukin-7 Receptor alpha chain (IL-7Rα). CD127 associates with CD132 (common γ chain) to form a high-affinity signaling IL-7R complex that mediates the biological effects of IL-7. CD127 can also complex with the Thymic Stromal Lymphopoietin Receptor (TSLPR) subunit to bind and mediate cellular responses to TSLP. CD127 is a 65-75 kDa type-I transmembrane protein that belongs to the hematopoietin receptor and the Ig gene superfamilies. Mice lacking CD127 display profoundly impaired development of the B and T lymphoid cell lineages, but display no obvious nonlymphoid abnormalities. IL-7Rα is expressed on common lymphoid progenitors and early stages of B lineage development in the bone marrow, on the earliest thymocyte progenitors, on CD4-CD8- double-negative and CD4+ and CD8+ single-positive thymocytes, and on most peripheral T lymphocytes. Intestinal intraepithelial lymphocytes with low-density γδ TCR expression upregulate CD127 expression in response to IL-2, which may be secreted by neighboring αβ TCR+ T cells.

      552543 Rev. 2
      Format Details
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      496 nm, 566 nm
      576 nm
      552543 Rev.2
      Citations & References
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      View product citations for antibody "552543" on CiteAb

      Development References (10)

      1. Akashi K, Kondo M, Weissman IL. Role of interleukin-7 in T-cell development from hematopoietic stem cells. Immunol Rev. 1998; 165:13-28. (Biology). View Reference
      2. Brugnera E, Bhandoola A, Cibotti R, et al. Coreceptor reversal in the thymus: signaled CD4+8+ thymocytes initially terminate CD8 transcription even when differentiating into CD8+ T cells. Immunity. 2000; 13(1):59-71. (Biology). View Reference
      3. Faust EA, Saffran DC, Toksoz D, Williams DA, Witte ON. Distinctive growth requirements and gene expression patterns distinguish progenitor B cells from pre-B cells. J Exp Med. 1993; 177(4):915-923. (Biology). View Reference
      4. Fujihashi K, Kawabata S, Hiroi T, et al. Interleukin 2 (IL-2) and interleukin 7 (IL-7) reciprocally induce IL-7 and IL-2 receptors on gamma delta T-cell receptor-positive intraepithelial lymphocytes. Proc Natl Acad Sci U S A. 1996; 93(8):3613-3618. (Biology). View Reference
      5. Goodwin RG, Friend D, Ziegler SF et al. Cloning of the human and murine interleukin-7 receptors: demonstration of a soluble form and homology to a new receptor superfamily. Cell. 1990; 60(6):941-951. (Biology). View Reference
      6. Henderson AJ, Narayanan R, Collins L, Dorshkind K. Status of kappa L chain gene rearrangements and c-kit and IL-7 receptor expression in stromal cell-dependent pre-B cells. J Immunol. 1992; 149(6):1973-1979. (Biology). View Reference
      7. Kouro T, Medina KL, Oritani K, Kincade PW. Characteristics of early murine B-lymphocyte precursors and their direct sensitivity to negative regulators. Blood. 2001; 97(9):2708-2715. (Biology). View Reference
      8. Noguchi M, Nakamura Y, Russell SM, et al. Interleukin-2 receptor gamma chain: a functional component of the interleukin-7 receptor. Science. 1993; 262(5141):1877-1880. (Biology). View Reference
      9. Peschon JJ, Morrissey PJ, Grabstein KH, et al. Early lymphocyte expansion is severely impaired in interleukin 7 receptor-deficient mice. J Exp Med. 1994; 180(5):1955-1960. (Biology). View Reference
      10. Yamashita Y, Oritani K, Miyoshi EK, Wall R, Bernfield M, Kincade PW. Syndecan-4 is expressed by B lineage lymphocytes and can transmit a signal for formation of dendritic processes. J Immunol. 1999; 162(10):5940-5948. (Clone-specific). View Reference
      View All (10) View Less
      552543 Rev. 2

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