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Purified Rat Anti-Mouse IL-12 p70
Product Details
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BD Pharmingen™
Mouse (QC Testing)
Rat IgG2b
CHO-Expressed Recombinant Mouse IL-12 p70
ELISA (Routinely Tested)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

ELISA Capture: IL-12 p70 ELISA: The purified 9A5 antibody (Cat. No. 554658) is useful as a capture antibody for a sandwich ELISA for specifically measuring mouse IL-12 p70 protein levels. Purified 9A5 antibody can be paired with the biotinylated C17.8 antibody (Cat. No. 554476) as the detection antibody, with recombinant mouse IL-12 p70 (Cat. No. 554592) as the standard. The purified 9A5 antibody should be titrated to determine its optimal concentration for ELISA capture (2 to 4 µg/ml). To obtain linear standard curves, doubling dilutions of mouse IL-12 ranging from ~2000 - 15 pg/ml are recommended for inclusion in each ELISA pair. For specific methodology, see BD Pharmingen's protocols or the chapter on ELISA in the Immune Function Handbook posted on the website at Caution: Use TMB substrate for higher substrate signal and higher sensitivity. For testing IL-12 p70 in serum or plasma, the OptEIA™ set (Cat. No. 555256) is recommended.

Note 1: This ELISA pair shows no cross-reactivity with any of the cytokines tested (e.g., mouse IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-15, GM-CSF, IFN-γ, MCP-1, TCA-3, TNF-α; human IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, G-CSF, GM-CSF, IFN-γ, lymphotactin, MCP-1, MCP-2, MIP-1α, MIP-1β, NT-3, PDGF-AA, sCD23, SCF, TNF-α, TNF-β, VEGF; rat IL-2, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, TNF-α).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
554658 Rev. 1
Antibody Details
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The 9A5 antibody reacts with the p70 subunit of mouse IL-12. The immunogen used to generate the 9A5 hybridoma was CHO-expressed recombinant mouse IL-12 p70.

This antibody is routinely tested by ELISA Capture. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

554658 Rev. 1
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
554658 Rev.1
Citations & References
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View product citations for antibody "554658" on CiteAb
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554658 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.