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Purified Rat Anti-Mouse, Human, Pig TGF-β1
Product Details
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BD Pharmingen™
Human (QC Testing), Human,Mouse,Pig (Tested in Development)
Rat IgG2a, κ
Recombinant Mouse TGF-β1
ELISA (Routinely Tested), Western blot (Reported)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

1. ELISA Capture: Purified A75-2 antibody is useful as the capture antibody in a sandwich ELISA for measuring mouse, human or pig TGF-β1 along with biotinylated anti-TGF-β1 clone A75-3 (Cat. No. 555053) as the detection antibody. The purified antibody should be titrated from 1.0 - 4.0 µg/ml to determine optimal concentration for ELISA capture. For specific methodology, visit the protocols section or the chapter on ELISA in the Immune Function Handbook, both of which can be found at This ELISA pair is recommended for measuring cytokine from experimental cell culture systems and is not recommended for testing serum or plasma samples. For measuring TGF-β1 in serum or plasma the BD OptEIA™ Human TGF-β1 ELISA Set (Cat. No. 559119) is specially formulated and recommended.

Important: Prior to ELISA assay for TGF- β1 protein levels, samples of biological fluids must be treated by acidification. The protocol is described below.

For serum samples, first dilute serum in PBS 1:5 (20 µl serum + 80 µl PBS), then add 1 N HCl in 1:25 to adjust to pH3 (i.e. 4 µl 1 N HCl to 100 µl diluted serum). Cell supernatants can be treated undiluted with 1 N HCl in 1:25 to adjust to pH3 (i.e. 4 µl 1 N HCl to 100 µl supernatant) Incubate acidified samples for 60 minutes at 4°C (alternatively, samples can be treated at room temperature for 15 minutes). After incubation neutralize the samples by treating each sample with 1 N NaOH 1:25 and test immediately or store at -70°C until testing.

Note: This ELISA pair shows no cross-reactivity with any of the cytokines tested (e.g., mouse IL-1β, IL-2, IL-3, IL-4, IL-6, IL-7, IL-9, IL-10, IL-12 p70, IL-15, GM-CSF, IFN-γ, MCP-1, TCA-3, TNF; human IL-1α, IL-1β , IL-2 , IL-3 , IL-4, IL-6, IL-7 , IL-8, IL9 , IL-10, IL-11 , IL-12 p70, IL-12 p40, IL-13, IL-15, G-CSF, GM-CSF, IFN-γ, lymphotactin, MCP-1, MCP-2, MIP-1α, MIP-1β, NT-3, PDGF-AA, sCD23, SCF, TNF, LT-α, VEGF; rat IL-2, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, TNF).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
555052 Rev. 1
Antibody Details
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The A75-2 antibody reacts with mouse, pig, human TGF-β1. The immunogen used to generate this hybridoma was recombinant mouse TGF-β1.

This antibody is routinely tested by ELISA Capture. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

555052 Rev. 1
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
555052 Rev.1
Citations & References
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View product citations for antibody "555052" on CiteAb
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555052 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.