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HRP Goat Anti-Rat Ig
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BD Pharmingen™ HRP Goat Anti-Rat Ig

Clone Polyclonal

Product Details
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BD Pharmingen™
Rat (QC Testing)
Goat Ig
ELISA (Routinely Tested)
Aqueous buffered solution containing BSA and ProClin™ 150 as preservative.

Preparation And Storage

Store undiluted at -20°C. Avoid multiple freeze-thaws of product. The polyclonal antibody was purified from antiserum by negative adsorption and affinity chromatography. The antibody was conjugated with HRP under optimum conditions, and unconjugated antibody and free HRP were removed.

The antibody solution was purified from goat antiserum and was passed through solid-phase immunoadsorbent gels to minimize cross-reactivity with mouse, human, cow, and horse serum proteins (multiple adsorption).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. ProClin is a trademark of Rohm and Haas Company.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Please refer to for technical protocols.
554017 Rev. 14
Antibody Details
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HRP Goat Anti-Rat Ig is a polyclonal secondary antibody used for the detection of rat Ig.  This antibody is tested by ELISA.  Investigators are highly encouraged to titrate the reagent for specific applications  (1:1000 dilution is the suggested starting dilution). It is reactive in ELISA with rat IgG, IgM, and IgA; a weaker signal may be detected on the rat IgG2b and IgM isotypes. It has some cross-reactivity with mouse IgG and no cross-reactivity with human Ig.  For immunohistochemical staining (IHC) of acetone-fixed frozen and paraffin-embedded sections, the use of biotinylated goat anti-rat Ig (Cat. No. 559286) is recommended. However, when using rat IgG2b primary antibodies, biotinylated mouse anti-rat IgG2b mAb G15-337 (Cat. No. 550327) is recommended as second step in IHC. When using rat IgM primary antibodies in IHC, biotinylated anti-rat and -hamster Ig κ light chain mAb RG7/7.6 (Cat. No. 550336) or biotinylated anti-rat IgM mAb G53-238 (Cat. No. 550330) as second step is recommended.

Warning:  HRP Goat Anti-Rat Ig (Cat. No. 554017) contains 0.004% (w/w) of a CMIT/MIT mixture (3:1), which is a mixture of: 5-chloro-2-methyl-4-isothiazolin-3-one [EC No 247-500-7] and 2-methyl-4-isothiazolin-3-one [EC No 220-239-6] (3:1).

Hazard statements

May cause an allergic skin reaction.

Precautionary statements

Wear protective gloves / eye protection.

Wear protective clothing.

Avoid breathing mist/vapours/spray.

If skin irritation or rash occurs: Get medical advice/attention.

IF ON SKIN: Wash with plenty of water.

Dispose of contents/container in accordance with local/regional/national/international regulations.

554017 Rev. 14
Format Details
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Horseradish Peroxidase is an enzyme that is capable of cleaving a substrate into hydrogen peroxide which can then be detected in solution or in situ. Antibodies, avidins, and other specific binding molecules conjugated with horseradish peroxidase, in conjunction with an appropriate substrate system, can be used for multiple ELISA or Immunohistochemical applications.
554017 Rev.14
Citations & References
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View product citations for antibody "554017" on CiteAb
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554017 Rev. 14

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.