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      Purified Rat Anti-Mouse Ly-6D
      Product Details
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      BD Pharmingen™
      Lymphocyte antigen 6 complex, locus D; Thb; Thymocyte B-cell antigen; Ly-61
      Mouse (QC Testing)
      Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2c, κ
      BALB/c mouse plasmacytoma MOPC-104E
      Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Cytotoxicity, Immunoprecipitation (Reported)
      0.5 mg/ml
      AB_396664
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      557360 Rev. 10
      Antibody Details
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      49-H4

      The 49-H4 antibody specifically binds to the ThB antigen (Ly-6D), a 15-kDa GPI-anchored protein of the Ly-6 Multigene Family. ThB has been detected on cortical thymocytes, small sIg- Thy-1- and sIg+ lymphoid cells in the bone marrow, thymic medullary epithelial cells, all epidermal layers (except the stratum corneum), and on peripheral B lymphocytes (but not peripheral T lymphocytes).  There is strain-to-strain variation in ThB antigen expression on splenic B cells. Strains with the Ly-6.2 haplotype (e.g., AKR, C57BL, DBA/2, SJL, SWR) stain more intensely with anti-ThB reagents than Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, NZB); B cells of Ly-6.1 and Ly-6.2 hybrids stain with intermediate intensity.  The proportion of ThB+ B cells to thymocytes do not differ significantly among strains.

      557360 Rev. 10
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      557360 Rev.10
      Citations & References
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      View product citations for antibody "557360" on CiteAb

      Development References (6)

      1. Boyd RL, Tucek CL, Godfrey DI. The thymic microenvironment. Immunol Today. 1993; 14(9):445-459. (Biology). View Reference
      2. Eckhardt LA, Herzenberg LA. Monoclonal antibodies to ThB detect close linkage of Ly-6 and a gene regulating ThB expression. Immunogenetics. 1980; 11(3):275-291. (Immunogen: Cytotoxicity). View Reference
      3. Godfrey DI, Izon DJ, Tucek CL, Wilson TJ, Boyd RL. The phenotypic heterogeneity of mouse thymic stromal cells. Immunology. 1990; 70(1):66-74. (Biology). View Reference
      4. Gumley TP, McKenzie IF, Kozak CA, Sandrin MS. Isolation and characterization of cDNA clones for the mouse thymocyte B cell antigen (ThB). J Immunol. 1992; 149(8):2615-2618. (Biology). View Reference
      5. Gumley TP, McKenzie IF, Sandrin MS. Polymorphism at the mouse Thb locus. Immunogenetics. 1994; 39(6):390-394. (Clone-specific: Immunoprecipitation). View Reference
      6. Matossian-Rogers A, Rogers P, Ledbetter JA, Herzenberg LA. Molecular weight determination of two genetically linked cell surface murine antigens: ThB and Ly-6. Immunogenetics. 1982; 15(6):591-599. (Clone-specific: Immunoprecipitation). View Reference
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      557360 Rev. 10

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.