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pig_mono_gran_PE_overlay.png

Flow cytometric analysis of anti-Pig Monocyte/Granulocyte staining on pig peripheral blood granulocytes. Pig whole blood was stained with a PE Mouse Anti-Pig Monocyte/Granulocyte antibody (Cat. No. 561499; solid line histogram) or with a PE Mouse IgG2b, κ Isotype Control (Cat. No. 559529; dashed line histogram). The erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable granulocytes. Flow cytometry was performed using a BD LSR™ II Flow Cytometry System.

pig_mono_gran_PE_overlay.png Red-Cap-Blue-Label.jpg

Flow cytometric analysis of anti-Pig Monocyte/Granulocyte staining on pig peripheral blood granulocytes. Pig whole blood was stained with a PE Mouse Anti-Pig Monocyte/Granulocyte antibody (Cat. No. 561499; solid line histogram) or with a PE Mouse IgG2b, κ Isotype Control (Cat. No. 559529; dashed line histogram). The erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable granulocytes. Flow cytometry was performed using a BD LSR™ II Flow Cytometry System.

Product Details
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BD Pharmingen™
SWC3; CD172a
Pig (QC Testing)
Mouse BALB/c IgG2b, κ
dd miniature swine thymocytes
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_10694894
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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561499 Rev. 1
Antibody Details
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74-22-15A

The 74-22-15A (switch variant of 74-22-15) monoclonal antibody, an isotype class-switch variant of mAb 74-22-15, specifically binds to a 230-kDa protein expressed by most pig macrophages, peripheral blood monocytes and granulocytes, and few lymphocytes.  mAb 74-22-15A does not crossreact with human or bovine cells.  This clone was clustered as anti-SWC3a at the First International Swine CD workshop.

561499 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
496 nm, 566 nm
576 nm
561499 Rev.1
Citations & References
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View product citations for antibody "561499" on CiteAb

Development References (8)

  1. Binns RM, Whyte A, Licence ST. The role of E-selectin in lymphocyte and polymorphonuclear cell recruitment into cutaneous delayed hypersensitivity reactions in sensitized pigs. J Immunol. 1996; 157(9):4094-4099. (Clone-specific: Immunohistochemistry). View Reference
  2. Blecha F, Kielian T, McVey DS. Workshop studies on monoclonal antibodies reactive against porcine myeloid cells. Vet Immunol Immunopathol. 1994; 43(1-3):269-272. (Biology). View Reference
  3. Haverson K, Bailey M, Higgins VR, Bland PW, Stokes CR. Characterization of monoclonal antibodies specific for monocytes, macrophages and granulocytes from porcine peripheral blood and mucosal tissues. J Immunol Methods. 1994; 170(2):233-245. (Biology). View Reference
  4. McCullough KC, Schaffner R, Natale V, Kim YB, Summerfield A. Phenotype of porcine monocytic cells: modulation of surface molecule expression upon monocyte differentiation into macrophages. Vet Immunol Immunopathol. 1997; 58(3-4):265-275. (Biology). View Reference
  5. Pescovitz MD, Lunney JK, Sachs DH. Preparation and characterization of monoclonal antibodies reactive with porcine PBL. J Immunol. 1984; 133(1):368-375. (Immunogen: Immunoprecipitation). View Reference
  6. Summerfield A, Haverson K, Thacker E, McCullough KC. Differentiation of porcine myeloid bone marrow haematopoietic cell populations. Vet Immunol Immunopathol. 2001; 80(1-2):121-129. (Biology). View Reference
  7. Sundt TM, Arn JS, Sachs DH. Patterns of T cell-accessory cell interaction in the generation of primary alloresponses in the pig. Transplantation. 1992; 54(5):911-916. (Clone-specific: Depletion). View Reference
  8. Yang H, Parkhouse RM. Phenotypic classification of porcine lymphocyte subpopulations in blood and lymphoid tissues. Immunology. 1996; 89(1):76-83. (Biology). View Reference
View All (8) View Less
561499 Rev. 1

 

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

Please refer to Support Documents for Quality Certificates

 

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.