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Flow cytometric analysis of ASGPR 1 expression on Human Hepatocellular Carcinoma (Hep G2) cells. Hep G2 cells (ATCC, HB-8065) were harvested using BD™ Accutase™ Cell Detachment Solution (Cat. No. 561527). The cells were stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 551436; dashed line histogram) or PE Mouse Anti-ASGPR 1 antibody (Cat. No. 563655; solid line histogram) at matched concentrations. Histograms were derived from gated events with the forward and side light scattering characteristics of viable Hep G2 cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Flow Cytometry System.
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BD Pharmingen™ PE Mouse Anti-ASGPR 1
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Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Accutase is a registered trademark of Innovative Cell Technologies, Inc.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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The 8D7 monoclonal antibody recognizes Asialoglycoprotein receptor 1 (ASGPR 1), also known as Hepatic lectin H1 (HL-1). ASGPR 1 is an approximately 42 kDA type II integral membrane protein that is expressed on the surface of hepatic cells. It is expressed by hepatocytes on the sinusoidal-lateral plasma membrane but not on the bile canalicular membrane. ASGPR 1 plays a role in serum glycoprotein homeostasis. It functions as a subunit of the Asialoglycoprotein receptor (ASGPR) complex that binds, internalizes, and transports various glycoproteins for lysosomal degradation. The receptor may also promote hepatic infection by the binding and uptake of various viruses. The immunogen used to generate the 8D7 hybridoma was rat liver membrane extracts. Rat ASGPR consists of three polypeptide subunits (Rat hepatic lectin 1-3 (RHL1-3). The 8D7 antibody has been shown to react with a subunit-specific epitope on RHL-1. Clone 8D7 cross-reacts with human ASGPR 1.

Development References (3)
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Mizuno M, Yamada G, Nagashima H. Development of a monoclonal antibody identifying an antigen which is segregated to the sinusoidal and lateral plasma membranes of rat hepatocytes. J Gastroenterol. 1986; 21(3):238-244. (Immunogen). View Reference
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Shimada M, Mizuno M, Uesu T, et al. A monoclonal antibody to rat asialoglycoprotein receptor that recognizes an epitope specific to its major subunit. Hepatol Res. 2003; 26(1):55-60. (Clone-specific: Flow cytometry, Immunofluorescence, Immunohistochemistry, Western blot). View Reference
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Touboul T, Hannan NR, Corbineau S, et al. Generation of functional hepatocytes from human embryonic stem cells under chemically defined conditions that recapitulate liver development. Hepatology. 2010; 51(5):1754-1765. (Biology). View Reference
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