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![BV510 Mouse Anti-Human CD41a](/content/dam/bdb/products/global/reagents/flow-cytometry-reagents/research-reagents/single-color-antibodies-ruo/563xxx/5632xx/563250_base/Hu_CD41a_BV510_Histo.png)
![BV510 Mouse Anti-Human CD41a](/content/dam/bdb/products/global/reagents/flow-cytometry-reagents/research-reagents/single-color-antibodies-ruo/563xxx/5632xx/563250_base/Hu_CD41a_BV510_Histo.png)
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![BV510 Mouse Anti-Human CD41a](/content/dam/bdb/products/global/reagents/flow-cytometry-reagents/research-reagents/single-color-antibodies-ruo/563xxx/5632xx/563250_base/Hu_CD41a_BV510_Histo.png)
Flow cytometric analysis of CD41a expression on human peripheral blood platelet. Platelets were isolated from fresh whole blood and fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655). After washing, the fixed platelets were stained with either BD Horizon™ BV510 Mouse IgG1, κ Isotype Control (Cat. No. 562946; dashed line histogram) or BD Horizon™ BV510 Mouse Anti-Human CD41a antibody (Cat. No. 563250; solid line histogram). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of platelets. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System
![](/content/dam/bdb/products/vialimages/packaging-images/black-vial-images/Green-Cap_Violet.png)
![BV510 Mouse Anti-Human CD41a](/content/dam/bdb/products/global/reagents/flow-cytometry-reagents/research-reagents/single-color-antibodies-ruo/563xxx/5632xx/563250_base/Hu_CD41a_BV510_Histo.png)
BD Horizon™ BV510 Mouse Anti-Human CD41a
![](/content/dam/bdb/products/vialimages/packaging-images/black-vial-images/Green-Cap_Violet.png)
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Brilliant Violet™ 510 is a trademark of Sirigen.
Companion Products
![sampleImage/](/content/dam/bdb/products/global/reagents/cell-preparation-separation-reagents/blood-lysis/staining-and-cell-preparation/554xxx/5546xx/554656_base/554656_01.png?imwidth=320)
![sampleImage/](/content/dam/bdb/products/global/reagents/flow-cytometry-reagents/research-reagents/buffers-and-supporting-reagents-ruo/554xxx/5546xx/554655_base/554655_01.png?imwidth=320)
![sampleImage/](/content/dam/bdb/products/vialimages/packaging-images/black-vial-images/Green-Cap_Violet.png?imwidth=320)
The HIP8 monoclonal antibody specifically binds to the α-chain of CD41. CD41 is also known as Integrin αIIb or Platelet GPIIb. The calcium-dependent complex of CD41 and CD61 (β3 integrin or GPIIIa) is normally expressed on platelets and megakaryocytes. The CD41/CD61 complex is the receptor for fibrinogen, fibronectin and von Willebrand factor, and mediates platelet adhesion and aggregation. CD41 (clone HIP8) completely inhibits ADP-, epinephrine-, and collagen-induced platelet activation, and partially inhibits ristocetin- and thrombin-induced platelet activation. This antibody is useful in the morphological and physiological studies of platelets and megakaryocytes.
The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon™ Brilliant Violet™ family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon™ BV510 can be excited by the violet laser and detected in the BD Horizon™ V500 (525/50-nm) filter set. BD Horizon™ BV510 conjugates are useful for the detection of dim markers off the violet laser.
![altImg](/content/dam/bdb/products/vialimages/packaging-images/formatimages/bv510_spectrum_guide.jpg)
Development References (2)
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von dem Borne AEGKr, Modderman PW, Admiraal LG, Nieuwenhuis, HK. Platelet antibodies, the overall results. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:951-966.
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von dem Borne AEGKr, Modderman PW. Cluster report: CD41. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:997-999.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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