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Biotin Rat Anti-Mouse IgM
Product Details
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BD Pharmingen™
Mouse (QC Testing)
Rat IgG2a, κ
Not reported
ELISA Detection (Routinely Tested), Flow cytometry (Tested During Development)
0.5 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Recommended Assay Procedures

Flow cytometry: For flow cytometric detection of intracytoplasmic IgM, FITC anti-mouse IgM (clone II/41) (Cat. No. 553437) is recommended.

ELISA: This antibody, coupled with avidin-HRP (Cat.No. 554058), may be used at ~2 µg/ml as the detection antibody for the detection of mouse IgM by indirect ELISA. For the detection of mouse IgM by sandwich ELISA, purified rat anti-mouse IgM (clone II/41) (Cat. No. 553435) is recommended for capture coupled with  biotin-conjugated rat anti-mouse IgM (clone R6-60.2) (Cat. No. 553406) and avidin-HRP (Cat.No. 554058) for detection.  Purified mouse IgM (Cat.No. 553472) may be used as the ELISA standard for either the indirect or sandwich ELISA.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
553436 Rev. 11
Antibody Details
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The II/41 clone has been reported to react specifically with mouse IgM of Igh-C[a] and Igh-C[b] haplotypes. It has been reported not to react with other Ig isotypes. In addition, the II/41 clone has been reported not to stimulate B-cell proliferation.

553436 Rev. 11
Format Details
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Biotin is a ubiquitous co-factor (also known as Vitamin B7) that has many properties that make it extremely useful for molecular biology. Biotin has an extremely high affinity for the Avidin family of proteins (Kd = 10-15 M), making it the perfect tool to link two molecules. Biotin labeled antibodies can be combined with any number of Avidin-conjugated probes in order to customize an assay to a particular need. This is especially useful in the case of magnetic cell separation using streptavidin/magnetic bead conjugates, or in the case of flow cytometry using streptavidin/fluorophore conjugates.
553436 Rev.11
Citations & References
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Development References (1)

  1. Laszlo G, Hathcock KS, Dickler HB, Hodes RJ. Characterization of a novel cell-surface molecule expressed on subpopulations of activated T and B cells. J Immunol. 1993; 150(12):5252-5262. (Biology). View Reference
553436 Rev. 11

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.