BD Pharmingen™ Alexa Fluor® 647 Mouse Anti-Human Pax-6
Clone O18-1330 (RUO)
Analysis of Pax-6 in H9 human embryonic stem cell derived Neural Stem Cells (NSC). H9 (WiCell, Madison, WI) derived NSC were harvested, fixed in BD Cytofix™ Fixation buffer (Cat. No. 554655), and permeabilized with BD Phosflow™ Perm buffer III (Cat. No. 558050). Cells were stained with matching concentrations of either Alexa Fluor® 647 Mouse IgG2a, κ isotype control (dashed histograms, Cat. No. 558053) or Alexa Fluor® 647 Mouse Anti-Human Pax-6 antibody (Cat. No. 562249, solid lines). Histograms were derived from gated events based on light scattering characteristics for the NSC. Flow cytometry was performed on a BD™ LSR II flow cytometry system.
Analysis of Pax-6 in H9 human embryonic stem cell derived Neural Stem Cells (NSC). H9 (WiCell, Madison, WI) derived NSC were harvested, fixed in BD Cytofix™ Fixation buffer (Cat. No. 554655), and permeabilized with BD Phosflow™ Perm buffer III (Cat. No. 558050). Cells were stained with matching concentrations of either Alexa Fluor® 647 Mouse IgG2a, κ isotype control (dashed histograms, Cat. No. 558053) or Alexa Fluor® 647 Mouse Anti-Human Pax-6 antibody (Cat. No. 562249, solid lines). Histograms were derived from gated events based on light scattering characteristics for the NSC. Flow cytometry was performed on a BD™ LSR II flow cytometry system.
Product Details
BD Pharmingen™
Oculorhombin, Aniridia type II protein, PAX6, AN2
Human (QC Testing)
Mouse BALB/c IgG2a, κ
Human Pax-6 aa 406-422 Peptide
Intracellular staining (flow cytometry) (Routinely Tested)
5 µl
5080
AB_11152956
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- All other brands are trademarks of their respective owners.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Fixation Buffer RUO
Size
100 mL
Cat No.
554655
Perm Buffer III RUO
Size
125 mL
Cat No.
558050
Alexa Fluor® 647 Mouse IgG2a, κ Isotype Control RUO
Size
50 Tests
Cat No.
558053
562249 Rev. 2
Antibody Details
O18-1330
Pax-6 is a member of the paired box (pax) gene family whose protein products are transcription factors involved in development. Pax family members share a highly conserved DNA binding domain that contains six alpha helices (paired domain) and a homeo box domain. Pax-6 has important roles in the development of the eye, nose, central nervous system, and pancreas. Defects in Pax-6 are responsible for various eye malformations including aniridia and Peters anomaly.
The O18-1330 monoclonal antibody reacts with human Pax-6. Because the Pax-6 protein sequence is highly conserved among vertebrate species, cross-reactivity with other species is possible.
562249 Rev. 2
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
562249 Rev.2
Citations & References
View product citations for antibody "562249" on CiteAb
Development References (4)
-
Cerf ME. Transcription factors regulating beta-cell function. Eur J Endocrinol. 2006; 155(5):671-679. (Biology). View Reference
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Chambers SM, Fasano CA, Papapetrou EP, Tomishima M, Sadelain M, Studer L. Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling. Nat Biotechnol. 2009; 27(3):275-280. (Methodology). View Reference
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Glaser T, Walton DS, Maas RL. Genomic structure, evolutionary conservation and aniridia mutations in the human PAX6 gene. Nat Genet. 1992; 2:232-239. (Biology). View Reference
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Osakada F, Jin ZB, Hirami Y, et al. In vitro differentiation of retinal cells from human pluripotent stem cells by small-molecule induction. J Cell Sci. 2009; 122:3169-3179. (Methodology). View Reference
562249 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.