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BD Pharmingen™ Human Intracellular Cytokine Staining Starter Kit
(RUO)Human Intracellular Cytokine Staining Starter Kit
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Development References (16)
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Andersson, U and J Andersson. D Fradelizie and D Emelie, ed. Cytokine Producing Cells. Paris: INSERM; 1994:32-49.
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Assenmacher M, J Schmitz and A Radbruch. Flow cytometric determination of cytokines in activated murine T helper lymphocytes: Expression of interleukin-10 in interferon-γ and in interleukin-4-expressing cells. Eur J Immunol. 1994; 24:1097-1101. (Biology).
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Austrup F, Vestweber D, Borges E, et al. P- and E-selectin mediate recruitment of T-helper-1 but not T-helper-2 cells into inflammed tissues. Nature. 1997; 385(6611):81-83. (Biology). View Reference
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Carter, L L, and S L Swain. Single cell analyses of cytokine production. Curr Opin Immunol. 1997; 9:177-182. (Biology).
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Elson LH, Nutman TB, Metcalfe DD, Prussin C. Flow cytometric analysis for cytokine production identifies T helper 1, T helper 2, and T helper 0 cells within the human CD4+CD27- lymphocyte subpopulation. J Immunol. 1995; 154(9):4294-4301. (Biology). View Reference
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Fujiwara, T, K Oda, S Yokota, S Takatsuki and Y Ikehara. Brefeldina A causes disassembly of the Golgi complex and accumulation of secretory proteins in the endoplasmic reticulum. J Biol Chem 263:18545. 1988. (Biology).
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Jung T, Schauer U, Heusser C, Neumann C, Rieger C. Detection of intracellular cytokines by flow cytometry. J Immunol Methods. 1993; 159(1-2):197-207. (Biology). View Reference
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Lecoeur, H, E Ledru, M-L Gougeon. A cytofluormetric method for the simultaneous detection of both intracellular and surface antigens of apoptotic peripheral lymphocytes. J Immunol Meth. 1998; 217:11-26. (Biology).
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Parks DR, Herzenberg LA, Herzenberg LA. Flow cytometry and fluorescence-activated cell sorting. In: Paul WE, ed. Fundamental Immunology, 2nd Edition. New York: Raven Press Ltd; 1989:781-802.
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Picker, L J, M K Singh, et al. Direct demonstration of cytokine synthesis heterogeneity amongh human memory/effector T-cells by flow cytometry. Blood. 1995; 86:1408-1419. (Biology).
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Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Biology). View Reference
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Sallusto F, Mackay CR, Lanzavecchia A. Selective expression of the eotaxin receptor CCR3 by human T helper 2 cells. Science. 1997; 277(5334):2005-2007. (Biology). View Reference
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Sander B, Hoiden I, Andersson U, Moller E, Abrams JS. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Methods. 1993; 166(2):201-214. (Biology). View Reference
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Sander, B, J Andersson and U Andersson. Assessment of cytokines by immunofluorescence and the paraformalde-hyde-saponin procedure. Immunol Rev. 1991; 119:65-93. (Biology).
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Shapiro, HM. Practical Flow Cytometry. (Biology).
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Vikingsson A, Pederson K, Muller D. Enumeration of IFN-gamma producing lymphocytes by flow cytometry and correlation with quantitative measurement of IFN-gamma. J Immunol Methods. 1994; 173(2):219-228. (Biology). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.