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V450 Mouse Anti-Human CD9
Product Details
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BD Horizon™
CD9 antigen (p24); 5H9; BA2; BTCC-1; DRAP-27; GIG2; MIC3; MRP-1; TSPAN29
Human
Mouse BALB/c IgG1, κ
Human C-ALL Cells
Flow cytometry
100 µg/mL
5 μL
III 617
928
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO (GMP)


Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

658167 Rev. 1
Antibody Details
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M-L13

The CD9 antibody, clone M-L13, is derived from the hybridization of mouse myeloma cells with spleen cells isolated from mice immunized with common acute lymphoblastic leukemia (cALL) cells.

The CD9 antibody recognizes a 24-kilodalton (kDa) cell surface glycoprotein belonging to the tetraspanin family. The CD9 antigen is also known as p24, tetraspanin-29 (Tspan-29), motility-related protein-1 (MRP-1), and leucocyte antigen MIC3.

658167 Rev. 1
Format Details
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V450
BD Horizon™ V450 Dye is part of the BD Horizon™ violet family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 405-nm and an emission maximum (Em Max) at 450-nm. BD Horizon™ V450, driven by BD innovation, is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 450-nm (e.g., a 450/50-nm bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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V450
Violet 405 nm
405 nm
450 nm
658167 Rev.1
Citations & References
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View product citations for antibody "658167" on CiteAb

Development References (18)

  1. Aoyama K, Oritani K, Yokota T, et al. Stromal cell CD9 regulates differentiation of hematopoietic stem/progenitor cells. Blood. 1999; 93:2586-2594. (Biology).
  2. Berditchevski F, Odintsova E. Characterization of integrin-tetraspanin adhesion complexes: role of tetraspanins in integrin signaling. J Biol Chem. 1999; 146:477-492. (Biology).
  3. Boucheix C, Benoit P, Krief P, et al. McMichael AJ, Beverley PCL, Cobbold S, et al, ed. Leucocyte Typing III: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1987:780-782.
  4. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  5. Clinical and Laboratory Standards Institute. 2005. (Biology).
  6. Johnson GD, MacLennan ICM. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:187-189.
  7. Maecker HT, Todd SC, Levy S. The tetraspanin superfamily: molecular facilitators. FASEB J. 1997; 11:428-442. (Biology).
  8. Ozaki Y, Satoh K, Kuroda K, et al. Anti-CD9 monoclonal antibody activates p72syk in human platelets. J Biol Chem. 1995; 270:15119-15124. (Biology).
  9. Powling MJ, Cox AD, Higgins J, Hardisty RM, Goodall AH. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:1012-1014.
  10. Slaper-Cortenbach ICM, Admiraal LG, von dem Borne AEG, van Leeuwen EF, Tetteroo PAT. McMichael AJ, Beverley PCL, Cobbold S, et al, ed. Leucocyte Typing III: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1987:480-484.
  11. Tai XG, Toyooka K, Yashiro Y, et al. CD9-mediated costimulation of TCR-triggered naive T cells leads to activation followed by apoptosis. J Immunol. 1997; 159:3799-3807. (Biology).
  12. Weir EG, Cowan K, LeBeau P, Borowitz MJ. A limited antibody panel can distinguish B-precursor acute lymphoblastic leukemia from normal B precursors with four color flow cytometry: implications for residual disease detection. Leukemia. 1999; 13:558-567. (Biology).
  13. Zeleznik NJ, Hollingsworth MA, Metzgar RS. McMichael AJ, Beverley PCL, Cobbold S, et al, ed. Leucocyte Typing III: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1987:389-392.
  14. Zola H, Swart B, Nicholson I, Voss E. Leukocyte and Stromal Cell Molecules: The CD Markers. 2007. (Biology).
  15. van Dongen JJ, Lhermitte L, Böttcher S, et al. EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012; 26(9):1908-1975. (Biology). View Reference
  16. von dem Borne AEG, Modderman PW. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:989-990.
  17. von dem Borne AEGKr, Admiraal LG, Daams M, Hogervorst F, Modderman PW. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:967-969.
  18. von dem Borne AEGKr, Modderman PW, Admiraal LG, Nieuwenhuis, HK. Platelet antibodies, the overall results. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:951-966.
View All (18) View Less
658167 Rev. 1

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