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Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Store vials at 2°C to 8°C. Conjugated forms should not be frozen and should be protected from exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.
CD154, clone 89-76, is derived from hybridization of mouse NS-1 cells with spleen cells from BALB/c mice immunized with sgp39-CD8 and activated human peripheral blood lymphocytes (PBLs). CD154 (CD40 ligand or CD40L) recognizes a 39-kilodalton (kDa) antigen that is a member of the tumor necrosis factor (TNF) receptor family. CD154 is transiently expressed on activated CD4 T lymphocytes. The expression of CD154 by activated T-helper cells triggers B-cell cycling through binding to CD40. This co-receptor interaction is required for B-lymphocyte maturation, response to T-dependent ligands, and isotype switching. T-lymphocyte proliferation and generation of cytotoxic T lymphocytes (CTLs) is associated with the presence of CD154. Defects in the CD154 gene are associated with the X-linked hyper IgM syndrome.
Development References (23)
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Chattopadhyay PK, Yu J, Roederer M. A live-cell assay to detect antigen-specific CD4+ T cells with diverse cytokine profiles.. Nat Med. 2005; 11(10):1113-7. (Biology). View Reference
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Ding L, Green JM, Thompson CB, Shevach EM. B7/CD28-dependent and -independent induction of CD40 ligand expression.. J Immunol. 1995; 155(11):5124-32. (Biology). View Reference
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Durie FH, Foy TM, Masters SR, Laman JD, Noelle RJ. The role of CD40 in the regulation of humoral and cell-mediated immunity.. Immunol Today. 1994; 15(9):406-11. (Biology). View Reference
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Frentsch M, Arbach O, Kirchhoff D, et al. Direct access to CD4+ T cells specific for defined antigens according to CD154 expression.. Nat Med. 2005; 11(10):1118-24. (Biology). View Reference
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Fuleihan R, Ahern D, Geha RS. CD40 ligand expression is developmentally regulated in human thymocytes.. Clin Immunol Immunopathol. 1995; 76(1 Pt 1):52-8. (Biology). View Reference
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Gauchat JF, Aubry JP, Disanto J, et al. Schlossman SF, Boumsell L, Gilks W, et al, ed. Leucocyte Typing V: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1995:416-418.
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Han S, Hathcock K, Zheng B, Kepler TB, Hodes R, Kelsoe G. Cellular interaction in germinal centers. Roles of CD40 ligand and B7-2 in established germinal centers.. J Immunol. 1995; 155(2):556-67. (Biology). View Reference
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Hollenbaugh D, Grosmaire LS, Kullas CD, et al. The human T cell antigen gp39, a member of the TNF gene family, is a ligand for the CD40 receptor: expression of a soluble form of gp39 with B cell co-stimulatory activity.. EMBO J. 1992; 11(12):4313-21. (Biology). View Reference
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Lane P, Traunecker A, Hubele S, Inui S, Lanzavecchia A, Gray D. Activated human T cells express a ligand for the human B cell-associated antigen CD40 which participates in T cell-dependent activation of B lymphocytes.. Eur J Immunol. 1992; 22(10):2573-8. (Biology). View Reference
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Noelle RJ, Roy M, Shepherd DM, Stamenkovic I, Ledbetter JA, Aruffo A. A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells. Proc Natl Acad Sci U S A. 1992; 89(14):6550-6554. (Biology). View Reference
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Noelle RJ. The role of gp39 (CD40L) in immunity.. Clin Immunol Immunopathol. 1995; 76(3 Pt 2):S203-7. (Biology). View Reference
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Roy M, Waldschmidt T, Aruffo A, Ledbetter JA, Noelle RJ. The regulation of the expression of gp39, the CD40 ligand, on normal and cloned CD4+ T cells. J Immunol. 1993; 151(5):2497-2510. (Biology). View Reference
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Spriggs MK, Armitage RJ, Strockbine L, et al. Recombinant human CD40 ligand stimulates B cell proliferation and immunoglobulin E secretion.. J Exp Med. 1992; 176(6):1543-50. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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