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FITC Mouse Anti-Human CD62L
Product Details
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BD™
L-selectin; LECAM-1; SELL; LNHR; LSEL; LAM-1; LEU8; PLNHR; TQ-1; MEL-14
Human
Mouse BALB/c IgG2a, κ
Human Peripheral Blood T Lymphocytes
Flow cytometry
12.5 μg/mL
20 μL
V S059
6402
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO (GMP)


Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

347443 Rev. 1
Antibody Details
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SK11

CD62L, clone SK11, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with peripheral blood T lymphocytes.

The CD62L antigen, Mr 80 kilodaltons (kd), is the leucocyte endothelial cellular adhesion molecule (LECAM). The CD62L antigen belongs to the selectin family of cell adhesion molecules. The CD62L molecule is the human homologue of the murine lymph node homing receptor, MEL 14.

347443 Rev. 1
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
347443 Rev.1
Citations & References
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Development References (14)

  1. Bevilacqua M, Butcher E, Furie B, et al. Selectins: a family of adhesion receptors. Cell. 1991; 67:233. (Biology).
  2. Camerini D, James SP, Stamenkovic I, Seed B. Leu-8/TQ1 is the human equivalent of the Mel-14 lymph node homing receptor. Nature. 1989; 342(6245):78-82. (Biology). View Reference
  3. Carbone A, Manconi R, Poletti A, Gloghini A, Paoli DE, Volpe R. Expression of Leu-8 surface antigen in B-cell lymphomas: correlation with other B-cell markers. J Pathol. 1988; 154:133. (Biology).
  4. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  5. Clinical and Laboratory Standards Institute. 2005. (Biology).
  6. Diacovo T, Springer TA. CD62L (L-selectin) cluster report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:1503-1504.
  7. Gatenby PA, Kansas GS, Xian CY, Evans RL, Engleman EG. Dissection of immunoregulatory subpopulations of T lymphocytes within the helper and suppressor sublineages in man. J Immunol. 1982; 129(5):1997-2000. (Biology). View Reference
  8. Kanof ME, James SP. Leu-8 antigen expression is diminished during cell activation but does not correlate with effector function of activated T lymphocytes. J Immunol. 1988; 140:3701-3706. (Biology).
  9. Kanof ME, Strober W, James SP. Induction of CD4 suppressor T cells with anti-Leu-8 antibody. J Immunol. 1987; 139(1):49-54. (Biology). View Reference
  10. Kansas GS, Wood GS, Engleman EG. Maturational and functional diversity of human B lymphocytes delineated with Anti-Leu-8. J Immunol. 1985; 134:3003-3006. (Biology).
  11. Kansas GS, Wood GS, Fishwild DM, Engleman EG. Functional characterization of human T lymphocyte subsets distinguished by monoclonal anti-leu-8. J Immunol. 1985; 134(5):2995-3002. (Biology). View Reference
  12. Lanier LL, Engleman EG, Gatenby P, Babcock GF, Warner NL, Herzenberg LA. Correlation of functional properties of human lymphoid cell subsets and surface marker phenotypes using multiparameter analysis and flow cytometry. Immun Rev. 1983; 74:143. (Biology).
  13. Lanier LL, Loken MR. Human lymphocyte subpopulations identified by using three-color immunofluorescence and flow cytometry analysis: correlation of Leu-2, Leu-3, Leu-7, Leu-8, and Leu-11 cell surface antigen expression. J Immunol. 1984; 132:151. (Biology).
  14. Michie SA, Garcia CF, Strickler JG, Dailey MO, Rouse RV, Warnke RA. Expression of the Leu-8 antigen by B-cell lymphomas. Am J Clin Pathol. 1987; 88(4):486-490. (Biology). View Reference
View All (14) View Less
347443 Rev. 1

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