Preparation And Storage
Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.
The CD44 antibody, clone L178, is derived from hybridization of Sp2/0 myeloma mouse cells with spleen cells from BALB/c mice immunized with TCR-γ/δ+ thymocytes. The CD44 (Leu-44) antibody recognizes a cell-surface glycoprotein that exists in a variety of isoforms. It is synthesized as a molecule with a molecular weight of 37 kilodaltons (kDa). Upon glycosylation, it is converted to an 80- to 95-kDa form. Alternatively, a 180- to 200-kDa form results from the addition of chondroitin sulfate.
Development References (11)
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Aruffo A, Stamenkovic I, Melnick M, Unterhill CB, Seed B. CD44 is the principal cell surface receptor for hyaluronate. Cell. 1990; 61:1303-1313. (Biology).
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Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
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Clinical and Laboratory Standards Institute. 2005. (Biology).
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Coombe DR, Rider CC. Lymphocyte homing receptors cloned: A role for anionic polysaccharides in lymphocyte adhesion. Immunol Today. 1989; 10:289. (Biology).
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Dalchau R. Knapp W, Dörken W, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford: Oxford University Press; 1989:622-625.
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Gallatin WM, Gale MJ Jr, Hoffman PA, et al. Selective replication of simian immunodeficiency virus in a subset of CD4+ lymphocytes. Proc Natl Acad Sci USA. 1989; 86:3301-3305. (Biology).
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Haynes BF, Telen MJ, Hale LP, Denning SM. CD44 – a molecule involved in leukocyte adherence and T-cell activation. Immunol Today. 1989; 10:423-428. (Biology).
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Picker LJ, de los Toyos J, Telen MJ, Haynes BF, Butcher EC. Monoclonal antibodies against the CD44 (In-[Lu]-related p80) and Pgp-1 antigens in man recognize the Hermes class of lymphocyte homing receptors. J Immunol. 1989; 142:2046-2051. (Biology).
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Shimizu Y, Van Seventer GA, Siraganian R, Wahl L, Shaw S. Dual role of the CD44 molecule in T-cell adhesion and activation. J Immunol. 1989; 143:2457-2463. (Biology).
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St John T, Meyer J, Idzerda R, Gallatin WM. Expression of CD44 confers a new adhesive phenotype on transfected cells. Cell. 1990; 60:45-52. (Biology).
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Stoll M, Dalchau R, Schmidt RE. Knapp W, Dörken W, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:619-622.
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