-
Your selected country is
Austria
- Change country/language
Old Browser
This page has been recently translated and is available in French now.
Looks like you're visiting us from {countryName}.
Would you like to stay on the current country site or be switched to your country?
BD FastImmune™ Antibody Cocktail (IFN-γ FITC, CD69 PE, CD8 PerCP-Cy™5.5, CD3 APC)
(RUO (GMP))Antibody Cocktail (IFN-γ FITC, CD69 PE, CD8 PerCP-Cy™5.5, CD3 APC)
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Description
Anti-Hu–IFN-γ, clone 25723.11, is derived from the hybridization of P3X-63-Ag8.653 mouse myeloma cells with lymph node cells from BALB/c mice immunized with recombinant human IFN-γ. CD69, clone L78, is derived from hybridization of Sp2/0-Ag14 mouse myeloma cells with lymph node cells from BALB/c mice immunized with a CD8+ alloantigen-directed cytotoxic T-lymphocyte (CTL) cell line. CD8, clone SK1, is derived from hybridization of NS-1 mouse myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood T lymphocytes. CD3, clone SK743-46, is derived from hybridization of NS-1 mouse myeloma cells with spleen cells from BALB/c mice immunized with human thymocytes. Anti-Human Interferon-γ (Anti-Hu–IFN-γ) recognizes a 20- to 25-kilodalton (kDa) glycoprotein. The CD69 antibody recognizes a very early human activation antigen. The CD69 antigen is a surface homodimer formed by the association of 28-kDa and 32-kDa chains that are held together by disulfide bridges. The CD8 antibody recognizes an antigen expressed on the 32-kilodalton (kDa) α subunit of a disulfide-linked bimolecular complex. The cytoplasmic domain of the α subunit of the CD8 antigen is associated with the protein tyrosine kinase p56lck. The CD8 molecule interacts with class I major histocompatibility complex (MHC) molecules resulting in increased adhesion between the CD8+ T lymphocytes and the target cells. Binding of the CD8 molecule to class I MHC molecules enhances the activation of resting T lymphocytes. The CD3 antibody reacts with the epsilon chain of the CD3 antigen/T-cell antigen receptor (TCR) complex. The antigen recognized by CD3 antibodies is noncovalently associated with either α/β or γ/δ TCR (70 to 90 kDa).
Preparation And Storage
Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.
Description | Clone | Isotype | EntrezGene ID |
---|---|---|---|
CD3 APC | SK7 | IgG1, κ | N/A |
IFN-γ FITC | TgMab-2 | IgG1, κ | N/A |
CD69 PE | 2D7/Basophils | IgG1, κ | N/A |
CD8 PerCP-CY5.5 | SK1 | IgG1, κ | N/A |
Development References (48)
-
Aggarwal B, Puri R. Human Cytokines: Their Role in Disease and Therapy. Cambridge, MA: Blackwell Science; 1995:3-24.
-
Asanuma H, Sharp M, Maecker HT, Maino VC, Arvin AM. Frequencies of memory T cells specific for varicella-zoster virus, herpes simplex virus, and cytomegalovirus determined by intracellular detection of cytokine expression. J Infec Dis. 2000; 181:859-866. (Biology).
-
Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
-
Chen JH, Prince H, Buck D, et al. Leu-23: an early activation antigen on human lymphocytes. Fed Proc. 1988; 2:A1214. (Biology).
-
Clevers H, Alarcón B, Wileman T, Terhorst C. The T cell receptor/CD3 complex: a dynamic protein ensemble. Annual Rev Immunol. 1988; 6:629. (Biology).
-
Clinical and Laboratory Standards Institute. 2005. (Biology).
-
ElGhazali GEB, Paulie S, Andersson G, et al. Number of interleukin-4– and interferon- γ –secreting human T cells reactive with tetanus toxoid and the mycobacterial antigen PPD or phytohemagglutindistinct response profiles depending on the type of antigen used for activation. Eur J Immunol. 1993; 23:2740-2745. (Biology).
-
Evans RL, Wall DW, Platsoucas CD, et al. Thymus-dependent membrane antigens in man: inhibition of cell-mediated lympholysis by monoclonal antibodies to TH2 antigen. Proc Natl Acad Sci U S A. 1981; 78(1):544-548. (Biology). View Reference
-
Gallagher PF, Fazekas de St. Groth B, Miller JFAP. CD4 and CD8 molecules can physically associate with the same T-cell receptor. Proc Natl Acad Sci USA. 1989; 86:10044-10048. (Biology).
-
Ghanekar SA, Nomura LE, Suni MA, Picker LJ, Maecker HT, Maino VC. γ interferon expression in CD8+ T cells is a marker for circulating cytotoxic T lymphocytes that recognize an HLA A2-restricted epitope of human cytomegalovirus phosphoprotein pp65. Clin Diagn Lab Immunol. 2001; 8:628-631. (Biology).
-
Hardy KJ, Sawada T. Human γ interferon strongly upregulates its own gene expression in peripheral blood lymphocytes. J Exp Med. 1989; 170:1021-1026. (Biology).
-
Haynes BF. Summary of T-cell studies performed during the Second International Workshop and Conference on Human Leukocyte Differentiation Antigens. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:3-30.
-
He XS, Rehermann B, Lopez-Labrador FX, et al. Quantitative analysis of hepatitis C virus-specific CD8+ T cells in peripheral blood and liver using peptide-MHC tetramers. Proc Natl Acad Sci USA. 1999; 96:5692-5697. (Biology).
-
Johnson HM, Bazer FW, Szente BE, Jarpe MA. How interferons fight disease. Scientific American. 1994; May:68-75. (Biology).
-
Kan EAR, Wang CY, Wang LC, Evans RL. Noncovalently bonded subunits of 22 and 28 kd are rapidly internalized by T cells reacted with Anti–Leu-4 antibody. J Immunol. 1983; 131:536-539. (Biology).
-
Karanikas V, Lodding J, Maino VC, McKenzie IFC. Flow cytometric measurement of intracellular cytokines detects immune responses in MUCI immunotherapy. Clin Cancer Res. 2000; 6:829-837. (Biology).
-
Kaul R, Dong T, Plummer FA, et al. CD8+ lymphocytes respond to different HIV epitopes in seronegative and infected subjects. J Clin Invest. 2001; 107:1303-1310. (Biology).
-
Kaul R, Plummer FA, Kimani J, et al. HIV-1-specific mucosol CD8+ lymphocyte responses in the cervix of HIV-1-resistant prostitues in Nairobi. J Immunol. 2000; 164:341-349. (Biology).
-
Kaul R, Rowland-Jones SL, Kimani J, et al. Late seroconversion in HIV-resistant Nairobi prostitutes despite pre-existing HIV-specific CD8+ responses. J Clin Invest. 2001; 107:341-349. (Biology).
-
Kern F, Faulhaber N, Fruömmel C, et al. Analysis of CD8 T cell reactivity to cytomegalovirus using protein-spanning pools of overlapping pentadecapeptides. Eur J Immunol. 2000; 30:1676-1682. (Biology).
-
Kern F, Surel IP, Brock C, et al T-cell epitope mapping by flow cytometry. Nat Med. 1998. (Biology).
-
Knowles RW. Immunochemical analysis of the T-cell–specific antigens. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:259-288.
-
Kotzin BL, Benike CJ, Engleman EG. Induction of immunoglobulin-secreting cells in the allogeneic mixed leukocyte reaction: regulation by helper and suppressor lymphocyte subsets in man. J Immunol. 1981; 127(9):931-935. (Biology). View Reference
-
Kuzushima K, Hoshino Y, Fufii K, et al. Rapid determination of Epstein-Barr–specific CD8+ T-cell frequencies by flow cytometry. Blood. 1999; 94:3094-3100. (Biology).
-
Lanier LL, Le AM, Phillips JH, Warner NL, Babcock GF. Subpopulations of human natural killer cells defined by expression of the Leu-7 (HNK-1) and Leu-11 (NK-15) antigens. J Immunol. 1983; 131(4):1789-1796. (Biology). View Reference
-
Ledbetter JA, Evans RL, Lipinski M, Cunningham-Rundles C, Good RA, Herzenberg LA. Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981; 153(2):310-323. (Biology). View Reference
-
Maecker HT, Dunn HS, Suni MA, et al. Use of overlapping peptide mixtures as antigens for cytokine flow cytometry. J Immunol Methods. 2001; 255:27-40. (Biology).
-
Maecker HT, Ghanekar SZ, Suni MA, HE X-S, Picker LJ, Maino VC. Factors affecting the efficiency of CD8+ T cell cross-priming with exogenous antigens. J Immunol. 2001; 166:7268-7275. (Biology).
-
Maino VC, Picker LJ. Identification of functional subsets by flow cytometry: intracellular detection of cytokine expression.. Cytometry. 1998; 34(5):207-15. (Biology). View Reference
-
Maino VC. Rapid assessment of antigen induced cytokine expression in memory T cells by flow cytometry.. Vet Immunol Immunopathol. 1998; 63(1-2):199-207. (Biology). View Reference
-
Moebius U. Knapp W, Dörken B, Gilks W, et al, ed. Leucocyte Typing IV. White Cell Differentiation Antigens. New York: Oxford University Press; 1989:342-343.
-
Nomura LE, Walker JM, Maecker HT. Optimization of whole blood antigen-specific cytokine assays for CD4+ T cells. Cytometry. 2000; 40:60-68. (Biology).
-
Openshaw P, Murphy EE, Hosken NA, et al. Heterogeneity of intracellular cytokine synthesis at the single-cell level in polarized T helper 1 and T helper 2 populations. J Exp Med. 1995; 182(5):1357-1367. (Biology). View Reference
-
Paliard X, Malefijt RDW, Yssel H, et al. Simultaneous production of IL-2, IL-4, and IFN-γ by activated human CD4+ and CD8+ T cell clones. J Immunol. 1988; 141:849-855. (Biology).
-
Powrie F, Coffman RL. Cytokine regulation of T-cell function: potential for therapeutic intervention. Immunol Today. 1993; 14:270-274. (Biology).
-
Reichert T, DeBruyere M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol. 1991; 60(2):190-208. (Biology). View Reference
-
Romagnani S, Del Prete G, Maggi E, et al. Human TH1 and TH2 subsets. Int Arch Allergy Immunol. 1992; 99:242-245. (Biology).
-
Rudd CE, Burgess KE, Barber EK, Schlossman SF. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:326-327.
-
Schmitz JE, Kuroda MJ, Santra S, et al. Control of viremia in simian immunodeficiency virus infection by CD8+ lymphocytes. Science. 1999; 238:857-860. (Biology).
-
Schwarting R, Biedobitek G, Stein H. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:428-432.
-
Street NE, Mosmann TR. Functional diversity of T lymphocytes due to secretion of different cytokine patterns. FASEB J. 1991; 5:171-176. (Biology).
-
Suni MA, Picker LJ, Maino VC. Detection of antigen-specific T cell cytokine expression in whole blood by flow cytometry.. J Immunol Methods. 1998; 212(1):89-98. (Biology). View Reference
-
Testi R, Philips J, Lanier LL. Constitutive expression of a phosphorylated activation (Leu-23) by CD3 bright thymocytes. J Immunol. 1988; 141:2257. (Biology).
-
Testi R, Philips JH, Lanier LL. Leu-23 induction as an early marker for functional CD3/T cell antigen receptor triggering: requirement for receptor cross-linking, prolonged elevation of intracellular (Ca ++ ), and stimulation of protein kinase C. J Immunol. 1988; 2:1214. (Biology).
-
Testi R, Phillips JH, Lanier LL. T cell activation via Leu-23 (CD69).. J Immunol. 1989; 143(4):1123-8. (Biology). View Reference
-
Testi R, Pulcinelli F, Frati L, Gazzaniga PP, Santoni A. CD69 is expressed on platelets and mediates platelet activation and aggregation.. J Exp Med. 1990; 172(3):701-7. (Biology). View Reference
-
Wood GS, Warner NL, Warnke RA. Anti–Leu-3/T4 antibodies react with cells of monocyte/macrophage and Langerhans lineage. J Immunol. 1983; 131(1):212-216. (Biology). View Reference
-
van Dongen JJM, Krissansen GW, Wolvers-Tettero ILM, et al. Cytoplasmic expression of the CD3 antigen as a diagnostic marker for immature T-cell malignancies. Blood. 1988; 71:603-612. (Biology).
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Although not required, these products are manufactured in accordance with Good Manufacturing Practices.