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Purified Mouse Anti-Mouse TREX1
Purified Mouse Anti-Mouse TREX1
Western blot analysis of TREX1 on a BC3H1 cell lysate (Mouse brain smooth muscle-like cells; ATCC CRL-1443).  Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10,000 dilution of the mouse anti-mouse TREX1 antibody.
Western blot analysis of TREX1 on a BC3H1 cell lysate (Mouse brain smooth muscle-like cells; ATCC CRL-1443).  Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10,000 dilution of the mouse anti-mouse TREX1 antibody.
Product Details
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BD Transduction Laboratories™
Mouse (QC Testing)
Mouse IgG1
Mouse TREX1 aa. 82-179
Western blot (Routinely Tested), Immunofluorescence (Not Recommended)
32 kDa
250 µg/ml
AB_399408
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611986 Rev. 1
Antibody Details
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29/TREX1

DNA replication, repair, and recombination requires the excision of nucleotides  from the DNA 3' termini. Many 3' to 5' exonucleases have been identified which catalyze the excision of monophosphates from the 3' termini of DNA. TREX1 and TREX2 are 3' to 5' exonucleases that contain three conserved exonuclease active site motifs (EASM) that may produce exonuclease activity. TREX1 and TREX2 are most closely related to the proofreading exonucleases of the bacterial  replicative DNA polymerases and the RNase T enzymes. Recombinant expression of TREX1 and TREX2 demonstrates that they have exonuclease activity when oligonucleotide is present. TREX1 shows the greatest exonuclease activity with partial duplex DNA, and no activity with single-stranded RNA or an RNA-DNA partial duplex. In addition, reconstitution of TREX1 with DNA polymerase β and DNA ligase III-XRCC1 facilitates accurate rejoining of a 3' mismatched base residue at a single-strand break. Thus, TREX1 and TREX2 are 3' to 5' exonucleases that may be important for excision of nucleotides during DNA replication, repair,  and recombination.

611986 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611986 Rev.1
Citations & References
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View product citations for antibody "611986" on CiteAb

Development References (2)

  1. Hoss M, Robins P, Naven TJ, Pappin DJ, Sgouros J, Lindahl T. A human DNA editing enzyme homologous to the Escherichia coli DnaQ/MutD protein. EMBO J. 1999; 18(13):3868-3875. (Biology). View Reference
  2. Mazur DJ, Perrino FW. Identification and expression of the TREX1 and TREX2 cDNA sequences encoding mammalian 3'-->5' exonucleases. J Biol Chem. 1999; 274(28):19655-19660. (Biology). View Reference
611986 Rev. 1

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