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Purified Mouse Anti-Human DLC-1
Purified Mouse Anti-Human DLC-1
Western blot analysis of DLC-1 on a SK-Hep-1 (human liver adenocarcinoma) lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti- human DLC-1 antibody.
Western blot analysis of DLC-1 on a SK-Hep-1 (human liver adenocarcinoma) lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti- human DLC-1 antibody.
Product Details
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG1
Human DLC-1 aa. 47-249
Western blot (Routinely Tested), Immunofluorescence (Not Recommended)
123 kDa
250 µg/ml
AB_399416
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at -20°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
612020 Rev. 1
Antibody Details
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3/DLC-1

Members of the Ras superfamily of GTPases are distantly related to the heterotrimeric G proteins and shuttle between inactive and active GTP-bound forms. Conversion between these forms is controlled by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). Rho is a GTPase that regulates cell morphology and motility in response to extracellular signals. p190A, p190B, and p122 RhoGAPs all contain RhoGAP domains and regulate the GTPase activity of Rho. p122 RhoGAP exhibits GAP activity for RhoA, but not Rac1, and binds and activates PLC-δ1. Microinjection of p122 RhoGAP suppresses LPA-induced formation of stress fibers and focal adhesions. Deleted in liver cancer-1 (DLC-1) was identified by its frequent deletion in liver cancer and is a human homologue of rat p122 RhoGAP. DLC-1 is expressed in many hepatocellular carcinoma tissues and cell lines and could be an important tumor suppressor gene. In normal human tissues, DLC-1 is expressed widely with the highest expression in heart and lung. Both p122 RhoGAP and DLC-1 have RhoGAP domains toward their C-terminal regions, which is thought to be important for regulation of Rho GTPase activity in many tissues.

This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

612020 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
612020 Rev.1
Citations & References
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View product citations for antibody "612020" on CiteAb

Development References (3)

  1. Homma Y, Emori Y. A dual functional signal mediator showing RhoGAP and phospholipase C-delta stimulating activities. EMBO J. 1995; 14(2):286-291. (Biology). View Reference
  2. Sekimata M, Kabuyama Y, Emori Y, Homma Y. Morphological changes and detachment of adherent cells induced by p122, a GTPase-activating protein for Rho. J Biol Chem. 1999; 274(25):17757-17762. (Biology). View Reference
  3. Yuan BZ, Miller MJ, Keck CL, Zimonjic DB, Thorgeirsson SS, Popescu NC. Cloning, characterization, and chromosomal localization of a gene frequently deleted in human liver cancer (DLC-1) homologous to rat RhoGAP. Cancer Res. 1998; 58(10):2196-2199. (Biology). View Reference
612020 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.