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Western blot analysis of CTCF on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-CTCF antibody.
Immunofluorescence staining of A431 cells (Human epithelial carcinoma; ATCC CRL-1555).
BD Transduction Laboratories™ Purified Mouse Anti-CTCF
BD Transduction Laboratories™ Purified Mouse Anti-CTCF
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Companion Products
The family of nucleic acid-binding C2H2 type zinc finger transcription factors is divided into two classes. One class consists of small proteins (Gli1, Krox-20, WT1, Egr-1, and Sp1) with conserved zinc finger clusters of 3 to 5 units, while the other class (ZNF91, ZNF74, ZFP37, CTCF) can contain more than 10 zinc finger clusters. CTCF is a ubiquitously expressed, highly conserved transcription factor that contains 10 C2H2- and 1 C2H-type zinc-finger motifs. CTCF binds to and represses transcription at the promoter-proximal regions of c-myc oncogenes, while CTCF can bind to and activate transcription at the promoter for amyloid β. Another mechanism of CTCF-mediated repression may include binding to insulator regions between enhancers and promoters resulting in enhancer blocking. In addition, CTCF gene may be a candidate tumor suppressor gene, since it localizes to a narrow cancer-associated chromosome region, and has been shown to have tumor-specific rearrangements in breast cancer patients. Thus, CTCF transcriptional regulation may be important for cell cycle progression, differentiation, apoptosis, and tumorigenesis.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (4)
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Bell AC, West AG, Felsenfeld G. The protein CTCF is required for the enhancer blocking activity of vertebrate insulators. Cell. 1999; 98(3):387-396. (Biology). View Reference
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Filippova GN, Fagerlie S, Klenova EM. An exceptionally conserved transcriptional repressor, CTCF, employs different combinations of zinc fingers to bind diverged promoter sequences of avian and mammalian c-myc oncogenes. Mol Cell Biol. 1996; 16(6):2802-2813. (Biology). View Reference
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Filippova GN, Lindblom A, Meincke LJ. A widely expressed transcription factor with multiple DNA sequence specificity, CTCF, is localized at chromosome segment 16q22.1 within one of the smallest regions of overlap for common deletions in breast and prostate cancers. Genes Chromosomes Cancer. 1998; 22(1):26-36. (Biology). View Reference
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Klenova EM, Nicolas RH, Paterson HF. CTCF, a conserved nuclear factor required for optimal transcriptional activity of the chicken c-myc gene, is an 11-Zn-finger protein differentially expressed in multiple forms. Mol Cell Biol. 1993; 13(12):7612-7624. (Biology). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.