BD Pharmingen™ Purified Rat Anti-Mouse IgE

Sandwich ELISA:  Purified rat anti-mouse IgE (clone R35-92) may be used at ~ 2 µg/ml as the capture antibody coupled with biotin rat anti-mouse IgE (clone R35-72) (Cat. No. 553414) as the detection antibody.  Researchers are strongly advised to titrate each reagent over a range of concentrations (e.g 1-8 µg/ml) for optimal results.  Purified mouse IgE (Cat. No. 557079, 553481, or 557080) may be used as the ELISA standard.  Researchers, however, are advised to first consider using purified rat anti-mouse IgE (clone R35-72) (Cat. No. 553413) and biotin rat anti-mouse IgE (clone R35-118) (Cat. No. 553419) as the ELISA sandwich pair for measuring mouse IgE where greater sensitivity is needed.  Alternatively, the BD OptEIA™ Mouse IgE ELISA Set (Cat. No. 555248) is offered as a convenient sandwich ELISA product that is easy-to-use and may be used for the quantitation of soluble mouse IgE.

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.