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BD Horizon™ V450 Streptavidin
Flow cytometric analysis of CD45R/B220 expression on mouse splenocytes using biotin-conjugated anti-mouse CD45R/B220 antibody and BD Horizon™ V450 Streptavidin. BALB/c mouse spleen cells were stained with Biotin anti-mouse CD45R/B220 (Cat. No. 553086) antibody followed by BD Horizon™ V450 Streptavidin (Cat. No. 560797; solid line of histogram) or with BD Horizon™ V450 Streptavidin alone (dashed line of histogram overlay). Flow cytometric histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSRII System.
Flow cytometric analysis of CD45R/B220 expression on mouse splenocytes using biotin-conjugated anti-mouse CD45R/B220 antibody and BD Horizon™ V450 Streptavidin. BALB/c mouse spleen cells were stained with Biotin anti-mouse CD45R/B220 (Cat. No. 553086) antibody followed by BD Horizon™ V450 Streptavidin (Cat. No. 560797; solid line of histogram) or with BD Horizon™ V450 Streptavidin alone (dashed line of histogram overlay). Flow cytometric histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSRII System.
Product Details
BD Horizon™
Flow cytometry (Routinely Tested)
0.5 mg/ml
AB_2033992
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. Streptavidin was conjugated with dye under optimum conditions, and unconjugated Streptavidin and free dye were removed
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- BD Horizon V450 has a maximum absorption of 406 nm and maximum emission of 450 nm. Before staining with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence.
- Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
560797 Rev. 3
Antibody Details
V450 Streptavidin is a useful second-step reagent for the indirect immunofluorescent staining of cells in combination with biotinylated primary antibodies for flow cytometric analysis.
Streptavidin is conjugated to BD Horizon V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406 nm and has an Em Max at 450 nm. Conjugates with BD Horizon V450 can be used in place of Pacific Blue™ conjugates.
560797 Rev. 3
Format Details
V450
BD Horizon™ V450 Dye is part of the BD Horizon™ violet family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 405-nm and an emission maximum (Em Max) at 450-nm. BD Horizon™ V450, driven by BD innovation, is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 450-nm (e.g., a 450/50-nm bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
V450
405 nm
450 nm
560797 Rev.3
Citations & References
View product citations for antibody "560797" on CiteAb
Development References (1)
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Shapiro HM. Practical Flow Cytometry, 3rd Edition. New York: Wiley-Liss, Inc; 1995:280-281.
560797 Rev. 3
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.