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Effects of anti-Integrin α5 chain (CD49e) antibodies on K562 cell binding to fibronectin. Aliquots of K562 cell suspension, an erythroleukemia cell line expressing Integrin α5 chain (CD49e) receptor, were incubated in the presence of the designated concentrations of Purified NA/LE Mouse Anti-Human Integrin α5 chain (CD49e) (Cat. No. 555614, • ) or Purified Mouse Anti-Human CD49e (Cat. No. 555651, □), for 30 minutes at room temperature. The suspensions were then transferred to plastic microtiter wells precoated with 12 µg/mL of fibronectin and incubated for 45 minutes at 37°C. Unattached cells were removed by aspiration and washing with PBS, and the attached cells were semi-quantitated using toluidine blue staining. The resulting absorbance signals are expressed relative to the average signal obtained without antibody.
BD Pharmingen™ Purified NA/LE Mouse Anti-Human Integrin α5 chain (CD49e)
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Preparation And Storage
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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
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The IIA1 monoclonal antibody specifically recognizes CD49e, a type I transmembrane glycoprotein which is also known as Integrin α5 Chain or VLA-5 α chain (VLA-5α). Following translation, CD49e is cleaved into two disulfide-linked chains of 135 and 25 kDa that associate with β1 integrin (CD29) to form the VLA-5 (Integrin α5/β1) complex. The VLA-5 complex is a well-established fibronectin receptor that is expressed on many cell types, including fibroblasts, endothelial cells, epithelial cells, platelets, thymocytes, T cells, B cells, the myeloid cell line U937, K562 and some melanoma cell lines. This clone inhibits α5β1 binding of ligands. It is useful for studies of the expression and function of this integrin.
Development References (1)
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Tran Van Nhieu G, Isberg RR. The Yersinia pseudotuberculosis invasin protein and human fibronectin bind to mutually exclusive sites on the alpha 5 beta 1 integrin receptor. J Biol Chem. 1991; 266(36):24367-24375. (Biology). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
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