The site is undergoing maintenance. Ordering can continue through fax and phone.
If you encounter problems while ordering. Please contact us at BDBCustomerService@bd.com
See More
BD Pharmingen™ Purified Mouse Anti-Human CD166
Clone 3A6 (RUO)
Profile of peripheral blood monocytes analyzed by flow cytometry.
Profile of peripheral blood monocytes analyzed by flow cytometry.
Product Details
BD Pharmingen™
Human (QC Testing)
Mouse IgG1, κ
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development)
0.5 mg/ml
VI A109
AB_397209
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Companion Products
Purified Mouse IgG1, κ Isotype Control RUO
Size
0.1 mg
Cat No.
555746
FITC Goat Anti-Mouse IgG/IgM RUO
Size
0.5 mg
Cat No.
555988
559260 Rev. 5
Antibody Details
3A6
The 3A6 monoclonal antibody specifically binds to the activated leukocyte cell adhesion molecule (ALCAM), a 100-105 kDa transmembrane glycoprotein, also known as the CD6 ligand, CD166. It is composed of an immunoglobulin-like extracellular domain, a transmembrane region and a short cytoplasmic tail. ALCAM belongs to the Ig superfamily of proteins and is expressed on neurons, activated T cells, activated monocytes, epithelial cells and fibroblasts. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T-cell development.
This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
559260 Rev. 5
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
559260 Rev.5
Citations & References
View product citations for antibody "559260" on CiteAb
Development References (3)
-
Aruffo A, Bowen MA, Patel DD, et al. CD6-ligand interactions: a paradigm for SRCR domain function?. Immunol Today. 1997; 18(10):498-504. (Biology). View Reference
-
Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
-
Patel DD, Wee SF, Whichard LP, et al. Identification and characterization of a 100-kD ligand for CD6 on human thymic epithelial cells. J Exp Med. 1995; 181(4):1563-1568. (Biology). View Reference
559260 Rev. 5
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.