PE Rat anti-Mouse Notch2

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BD Pharmingen™ PE Rat anti-Mouse Notch2

Name: PE Rat anti-Mouse Notch2
Brand: BD Pharmingen™
SKU: 562755

Clone 16F11

(RUO)

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Protocol Library

Scientific Resources

Multicolor flow cytometric analysis of Notch2 expression on BALB/c mouse bone marrow cells. Mouse bone marrow cells were stained with FITC Rat Anti-Mouse CD11b antibody (Cat. No. 553310/557396/561688) and either PE Rat IgG1, κ Isotype Control (Cat. No. 554685, Left Panel) or PE Rat Anti-Mouse Notch2 antibody (Cat. No. 562755, Right Panel). The two-color flow cytometric dot plots showing the expression of CD11b versus Notch2 (or Ig Isotype Control staining) were derived from gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometry was performed using a BD FACSCanto™ II System.

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Product Details

Brand: BD Pharmingen™

Alternative Name: Notch2; Notch 2; NOTC2; Motch B; N2

Reactivity: Mouse (QC Testing)

Isotype: Rat IgG1, κ

Immunogen: Mouse Notch2 extracellular domain Recombinant Protein

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

Entrez Gene ID: 18129

RRID: AB_2737771

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
An isotype control should be used at the same concentration as the antibody of interest.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

PE Rat IgG1, κ Isotype Control RUO

Size 0.1 mg Cat No. 554685

FITC Rat Anti-CD11b RUO

Size 0.5 mg Cat No. 553310

FITC Rat Anti-CD11b RUO

Size 0.1 mg Cat No. 557396

FITC Rat Anti-CD11b RUO

Size 25 µg Cat No. 561688

562755 Rev. 1

Antibody Details

16F11

The 16F11 monoclonal antibody specifically binds to the extracellular domain of Notch2. This Type 1 transmembrane protein is a member of the Notch family that includes Notch1-4. Notch ligands include membrane-bound Jagged1, Jagged2 and Delta-like1, 3 and 4. Notch receptors mediate juxtacrine signals between adjacent cells. Upon ligand binding, Notch2 undergoes proteolytic cleavage that results in the release of the Notch intracellular domain, NICD. NICD translocates to the nucleus where it forms a transcriptional activator complex with the RBP-J DNA binding factor and other transcriptional cofactors. These multimeric complexes regulate the expression of multiple genes including those that orchestrate many facets of embryonic development and the subsequent functioning of cells within multiple organ systems. Notch2 is expressed on bone marrow cells, DN1 and DN2 thymocytes, B cells and activated T cells. Notch2 plays multiple roles in the immune system including the development of marginal zone B cells and the regulation of granzyme B expression by CD8+ T cells. In altered forms, Notch2 has been implicated in cellular transformation or progression in various neoplasms.

562755 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

562755 Rev.1

Citations & References

Development References (4)

Fiorini E, Merck E, Wilson A, et al. Dynamic regulation of Notch1 and Notch2 surface expression during T cell development and activation revealed by novel monoclonal antibodies. J Immunol. 2009; 183(11):7212-7222. (Immunogen: Flow cytometry, Immunofluorescence). View Reference
Maekawa Y, Minato Y, Ishifune C, et al. Notch2 integrates signaling by the transcription factors RBP-J and CREB1 to promote T cell cytotoxicity. Nat Immunol. 2008; 9(10):1140-1147. (Biology). View Reference
Radtke F, Fasnacht N, Macdonald HR. Notch signaling in the immune system. Immunity. 2010; 32(1):14-27. (Biology). View Reference
Saito T, Chiba S, Ichikawa M, et al. Notch2 is preferentially expressed in mature B cells and indispensable for marginal zone B lineage development. Immunity. 2003; 18(5):675-685. (Biology). View Reference

View All (4)

562755 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.