The expression ICOS on activated T lymphocytes. BALB/c splenocytes were either unstimulated (top panels) or activated by culture for 48 hours in the presence of soluble 145-2C11 mAb (anti-mouse CD3e, Cat. No. 553057, bottom panels). Both sets of cells were stained with either PE-conjugated rat IgG2b, κ isotype control mAb A95-1 (Cat. No. 553989, left panels) or PE-conjugated mAb 7E.17G9 (right panels), then FITC-conjugated mAb 53-6.7 (anti-mouse CD8a, Cat. No. 553030/553031). Viable leukocytes were selected by exclusion of propidium iodide. Flow cytometry was performed on a FACSCalibur™ (BD Biosciences, San Jose, CA).
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The expression ICOS on activated T lymphocytes. BALB/c splenocytes were either unstimulated (top panels) or activated by culture for 48 hours in the presence of soluble 145-2C11 mAb (anti-mouse CD3e, Cat. No. 553057, bottom panels). Both sets of cells were stained with either PE-conjugated rat IgG2b, κ isotype control mAb A95-1 (Cat. No. 553989, left panels) or PE-conjugated mAb 7E.17G9 (right panels), then FITC-conjugated mAb 53-6.7 (anti-mouse CD8a, Cat. No. 553030/553031). Viable leukocytes were selected by exclusion of propidium iodide. Flow cytometry was performed on a FACSCalibur™ (BD Biosciences, San Jose, CA).
Product Details
BD Pharmingen™
ICOS; Ailim; CCLP; CRP-1; Ly115; Inducible T-cell costimulator
Mouse (QC Testing)
Rat IgG2b, κ
Mouse Icos cDNA and ICOS hexahistidine fusion protein
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_394349
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Companion Products
PE Rat IgG2b, κ Isotype Control RUO
Size
0.1 mg
Cat No.
553989
FITC Rat Anti-Mouse CD8a RUO
Size
0.1 mg
Cat No.
553030
552146 Rev. 1
Antibody Details
7E.17G9
The 7E.17G9 monoclonal antibody specifically binds to CD278, the Inducible Costimulatory molecule (ICOS), a 47-57 kDa homodimeric glycoprotein of the CD28 family of costimulatory molecules. ICOS is expressed on subpopulations of CD4-CD8- and CD4+CD8- (but not CD4-CD8+ or CD4+CD8+) thymocytes, on some T-cell lines, and on small numbers of peripheral leukocytes. It is upregulated on T lymphocytes following activation via the T-cell receptor. The T-cell activation marker H4 is the same molecule as ICOS. ICOS is a costimulatory receptor, and its ligand on antigen-presenting cells has been called B7RP-1, GL50, B7h, B7-H2, or LICOS. There is considerable evidence that the interaction of ICOS with its ligand is involved in the regulation of many, but not all, T-cell-mediated immune responses.
552146 Rev. 1
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
552146 Rev.1
Citations & References
View product citations for antibody "552146" on CiteAb
Development References (9)
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Buonfiglio D, Bragardo M, Redoglia V, et al. The T cell activation molecule H4 and the CD28-like molecule ICOS are identical. Eur J Immunol. 2000; 30(12):3463-3467. (Biology). View Reference
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Chambers CA. The expanding world of co-stimulation: the two-signal model revisited. Trends Immunol. 2001; 22(4):217-223. (Biology). View Reference
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Dong C, Temann UA, Flavell RA. Cutting edge: critical role of inducible costimulator in germinal center reactions. J Immunol. 2001; 166(6):3659-3662. (Biology). View Reference
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Gonzalo JA, Delaney T, Corcoran J, Goodearl A, Gutierrez-Ramos JC, Coyle AJ. Cutting edge: the related molecules CD28 and inducible costimulator deliver both unique and complementary signals required for optimal T cell activation. J Immunol. 2001; 166(1):1-5. (Biology). View Reference
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Mages HW, Hutloff A, Heuck C, et al. Molecular cloning and characterization of murine ICOS and identification of B7h as ICOS ligand. Eur J Immunol. 2000; 30(4):1040-1047. (Biology). View Reference
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McAdam AJ, Chang TT, Lumelsky AE, et al. Mouse inducible costimulatory molecule (ICOS) expression is enhanced by CD28 costimulation and regulates differentiation of CD4+ T cells.. J Immunol. 2000; 165(9):5035-40. (Immunogen). View Reference
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Schwartz RH. Immunology. It takes more than two to tango. Nature. 2001; 409(6816):31-32. (Biology). View Reference
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Sperling AI, Bluestone JA. ICOS costimulation: It's not just for TH2 cells anymore. Nat Immunol. 2001; 2(7):573-574. (Biology). View Reference
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Wallin JJ, Liang L, Bakardjiev A, Sha WC. Enhancement of CD8+ T cell responses by ICOS/B7h costimulation. J Immunol. 2001; 167(1):132-139. (Biology). View Reference
552146 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.