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Flow cytometric analysis of CD201 expression on bEND.3 cells. Cells from the mouse bEnd.3 (Endothelioma, ATCC CRL-2299) cell line were stained with either PE Rat IgG2b κ Isotype Control (Cat. No. 555848; dotted line histogram) or PE Rat Anti-Mouse CD201 antibody (Cat. No. 566337; solid line histogram) at 0.5 μg/test. Data shown on this Technical Data Sheet are not lot specific. The fluorescence histogram showing CD201 expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
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BD Pharmingen™ PE Rat Anti-Mouse CD201
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- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
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The 1560 monoclonal antibody specifically binds to CD201 which is also known as Endothelial protein C receptor (EPCR) or Activated protein C receptor (APC receptor). CD201 is encoded by Procr (Protein C receptor, endothelial). CD201 is a ~25 kDa single-pass type I transmembrane glycoprotein that binds both protein C and activated protein C (APC) and augments the activation of protein C by the thrombin-thrombomodulin/CD141 complex. APC functions as a serine protease that controls thrombin IIa generation by inhibiting factors Va and VIIIa. CD201 thus plays an essential role in preventing blood coagulation which may lead to tissue damage during sepsis and inflammatory responses. Procr-gene deletion reportedly leads to embryonic lethality due to placental thrombosis. CD201 is expressed by endothelial cells with higher expression on large blood vessels. The 1560 monoclonal antibody can reportedly be used for the detection of bone marrow hematopoietic stem cells (HSC) which express high levels of CD201. A soluble form of CD201 which can bind protein C and APC has also been described.

Development References (5)
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Balazs AB, Fabian AJ, Esmon CT, Mulligan RC. Endothelial protein C receptor (CD201) explicitly identifies hematopoietic stem cells in murine bone marrow.. Blood. 2006; 107(6):2317-21. (Clone-specific: Blocking, Flow cytometry, Fluorescence activated cell sorting, Functional assay). View Reference
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Crawley JT, Gu JM, Ferrell G, Esmon CT. Distribution of endothelial cell protein C/activated protein C receptor (EPCR) during mouse embryo development.. Thromb Haemost. 2002; 88(2):259-66. (Immunogen: Immunohistochemistry). View Reference
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Disse J, Petersen HH, Larsen KS, et al. The endothelial protein C receptor supports tissue factor ternary coagulation initiation complex signaling through protease-activated receptors.. J Biol Chem. 2011; 286(7):5756-67. (Clone-specific: Functional assay). View Reference
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Gu JM, Crawley JT, Ferrell G, et al. Disruption of the endothelial cell protein C receptor gene in mice causes placental thrombosis and early embryonic lethality.. J Biol Chem. 2002; 277(45):43335-43. (Clone-specific: Immunohistochemistry). View Reference
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Stearns-Kurosawa DJ, Kurosawa S, Mollica JS, Ferrell GL, Esmon CT. The endothelial cell protein C receptor augments protein C activation by the thrombin-thrombomodulin complex.. Proc Natl Acad Sci USA. 1996; 93(19):10212-6. (Biology). View Reference
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